Skip to main content Accessibility help
×
Home

Oxidative stress and changes in adenosine deaminase activity of cattle experimentally infected by Fasciola hepatica

  • ALEKSANDRO S. DA SILVA (a1) (a2), MATHEUS D. BALDISSERA (a3), NATHIELI B. BOTTARI (a2), MATHEUS E. GABRIEL (a4), LEANDRO A. RHODEN (a4), MANOELA M. PIVA (a4), RICARDO CHRIST (a4), FERNANDA A. STEDILLE (a4), ANDERSON GRIS (a4), VERA M. MORSCH (a2), MARIA ROSA SCHETINGER (a2) and RICARDO E. MENDES (a4)...

Summary

The aim of this study was to evaluate the oxidative stress in serum and liver and adenosine deaminase (ADA) activity of cattle experimentally infected by Fasciola hepatica. The group A consisted of five healthy animals (uninfected), and the group B was composed of five animals orally infected with 200 metacercariae of F. hepatica. On days 20, 40, 60 and 80 post-infection (PI) serum was collected to measure oxidative stress variables. On day 100 PI, animals were humanely euthanized and liver samples were collected. Infected animals showed lower (P < 0·05) seric ADA activities on days 40 and 60 PI but higher (P < 0·05) in the liver tissue compared with uninfected animals. Seric and hepatic reactive oxygen species (ROS) were higher (P < 0·05) in infected compared with uninfected animals. Hepatic thiobarbituric acid reactive substances were higher (P < 0·05) in infected animals. Catalase and glutathione S-transferase activities were lower in liver tissue of infected animals, while glutathione peroxidase was higher compared with uninfected (P < 0·05). In summary, we conclude that oxidative stress occurs in cattle experimentally infected by F. hepatica, mainly due to excessive ROS production in the course of fasciolosis, contributing to hepatic damage, and that increased in hepatic ADA activity may contribute to the inflammatory process.

Copyright

Corresponding author

*Corresponding authors: UDESC, Animal Science, Rua Beloni Trombeta Zanini, Chapecó 89805-030, Brazil. E-mail: aleksandro_ss@yahoo.com.br; ricardo.mendes@ifc-concordia.edu.br

References

Hide All
Abbracchio, M. P. and Ceruti, S. (2007). P1 receptors and cytokine secretion. Purinergic Signalling 3, 1325.
Aebi, H. (1984). Catalase in vitro . Methods in Enzymology 105, 121126.
Baldissera, M. D., Bottari, N. B., Mendes, R. E., Schwertz, C. I., Lucca, N. J., Dalenogare, D., Bochi, G. V., Moresco, R. N., Morsch, V. M., Schetinger, V. M., Rech, V. C., Jaques, J. A. and Da Silva, A. S. (2015). Activity of cholinesterases, pyruvate kinase and adenosine deaminase in rats experimentally infected by Fasciola hepatica: influence of these enzymes on inflammatory response and pathological findings. Pathology – Research and Practice 211, 871876.
Baldissera, M. D., Mendes, R. E., Doleski, P. H., Bottari, N. B., Casali, E. A., Cardoso, V. V., Henker, L. C., Christ, R., Stedille, F. A., Stefani, L. M. and Da Silva, A. S. (2016). Hepatic and seric levels of purines in rats experimentally infected by Fasciola hepatica . Parasitology Research 115, 23632369.
Bottari, N. B., Mendes, R. E., Lucca, N. J., Schwertz, C. I., Henker, L. C., Olsson, D. C., Piva, M. M., Sangoi, M., Campos, L. P., Moresco, R. N., Jaques, J. A. and Da Silva, A. S. (2015). Oxidative stress associated with pathological lesions in the liver of rats experimentally infected by Fasciola hepatica . Experimental Parasitology 159, 2428.
Bottari, N. B., Mendes, R. E., Baldissera, M. D., Bochi, G. V., Moresco, R. N., Schetinger, M. R., Christ, R., Gheller, L., Marques, E. J. and Da Silva, A. S. (2016). Relation between iron metabolism and antioxidants enzymes and δ-ALA-D activity in rats experimentally infected by Fasciola hepatica . Experimental Parasitology 165, 5863.
Box, G. E. P. (1953). Non-normality and tests on variances. Biometrika 40, 318335.
Bradford, M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein – dye binding. Analytical Biochemistry 72, 248254.
Carlberg, I. and Mannervik, B. (1985). Glutathione reductase. Methods in Enzymology 113, 484490.
Choi, C. Y., An, K. W. and An, M. I. (2008). Molecular characterization and mRNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder (Paralichthys olivaceus). Comparative Biochemistry and Physiology Part A: Molecular Integrative Physiology 149, 330337.
Colpo, E., de Bem, A. F., Pieniz, S., Schettert, S. D., dos Santos, R. M., Farias, I. L., Bertoncello, I., Moreira, C. M., Barbosa, N. V., Moretto, M. B. and Rocha, J. B. (2008). A single high dose of ascorbic acid and iron is not correlated with oxidative stress in healthy volunteers. Annals of Nutrition & Metabolism 53, 7985.
De Oliveira, R. B., Senger, M. R., Vasques, L. M., Gasparotto, J., Dos Santos, J. P., Pasqualli, M. A., Moreira, J. C. F., Silva, F. P. and Gelain, D. P. (2013). Schistosoma mansoni infection causes oxidative stress and alters receptor for advanced glycation endproducts (RAGE) and tau levels in multiple organs in mice. International Journal for Parasitology 43, 371379.
Doleski, P. H., Mendes, R. E., Leal, D. B., Piva, M. M., Da Silva, E. S., Gabriel, M. E., Lucca, N. J., Schwertz, C. I., Giacomim, P., Morsch, V. M., Schetinger, M. R., Baldissera, M. D. and Da Silva, A. S. (2016). Seric and hepatic NTPDase and 5′-nucleotidase activities of rats experimentally infected by Fasciola hepatica . Parasitology 143, 551556.
Fiss, L., Adrien, M. A., Marcolongo-Pereira, C., Assis-Brasil, N. D., Sallis, E. S. V., Riet-Correa, F., Ruas, J. L. and Schild, A. L. (2013). Subacute and acute fasciolosis in sheep in Southern Brazil. Parasitology Research 112, 883887.
Fredholm, B. B., Johansson, S. and Wang, Y. W. (2011). Adenosine and the regulation of metabolism and body temperature. Advances in Pharmacology 61, 7794.
Fujita, T. (2002). Formation and removal of reactive oxygen species, lipid peroxidation and free radicals, and their biological effects. Yakugaku Zasshi 122, 203218.
Giusti, G. and Gakis, C. (1971). Temperature conversion factors, activation energy, relative substrate specificity and optimum pH of adenosine deaminase from human serum and tissues. Enzyme 12, 417425.
Giusti, G. and Galanti, B. (1984). Colorimetric method. In Berg-meyer, H.U. Methods of Enzymatic Analysis, 3rd ed., Verlag Chemie, Weinheim, p. 315323.
Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 249, 71307139.
Halliwell, B. and Gutteridge, J. M. (1999). Free Radicals in Biology and Medicine. Clarendon Press, Oxford, pp. 1543.
Halliwell, B. and Gutteridge, J. M. C. (2007) Free Radicals in Biology and Medicine, 4th Edn. Oxford University Press, New York.
Jentzsch, A. M., Bachmann, H., Furts, P. and Biesalski, H. K. (1996). Improved analysis of malondialdehyde in human body fluids. Free Radical Biology and Medicine 20, 251256.
Kamel, H. H., Sarhan, R. M. and Saad, G. A. (2015). Biochemical assessment of oxidative stress versus liver enzymes in patients with chronic fascioliasis. Journal of Parasitic Diseases 39, 628633.
Khadija, A. A., Mohammed, S., Saad, A. M. and Mohamed, H. E. (2009). Response of broiler chicks to dietary monosodium glutamate. Pakistan Veterinary Journal 29, 165168.
Kilic, E., Yazar, S., Saraymen, R. and Ozbilge, H. (2003). Serum malondialdehyde level in patients infected with Ascaris lumbricoides . World Journal of Gastroenterology 9, 23322334.
Kolodziejczyk, L., Siemieniuk, E. and Skrzydlewska, E. (2005). Antioxidant potential of rat liver in experimental infection with Fasciola hepatica . Parasitology Research 96, 367372.
Kolodziejczyk, L., Siemieniuk, E. and Skrzydlewska, E. (2006). Fasciola hepatica: effects on the antioxidant properties and lipid peroxidation of rat serum. Experimental Parasitology 113, 4348.
Kolodziejczyk, L., Laszczynska, M., Masiuk, M., Grabowska, M. and Skrzydlewska, E. (2015). Immunoexpression of intermediate filaments and morphological changes in the liver and bile ducts of rats infected with Fasciola hepatica . Biotechnic and Histochemistry 90, 477485.
Lopaczyski, W. and Zeisel, S. H. (2001). Antioxidants, programmed cell death and cancer. Nutrition Research 21, 295307.
Lushchak, V. I. (2011). Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicology 101, 1330.
Mas-Coma, S., Bargues, M. D. and Valero, M. A. (2005). Fascioliasis and other plant-borne trematode zoonoses. International Journal for Parasitology 35, 12551278.
Mas-Coma, S., Bargues, M. D. and Valero, M. A. (2014). Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario. Parasitology 141, 19181946.
Mills, J. H., Kim, D. G., Krenz, A., Chen, J. F. and Boyne, M. S. (2012). A2A adenosine receptor signaling in lymphocytes and the central nervous system regulates inflammation during experimental autoimmune encephalomyelitis. Journal of Immunology 188, 57135722.
Misra, H. P. and Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry 247, 31703175.
Murphy, M. P., Holmgren, A., Larsson, N.G., Halliwell, B., Chang, C. J., Kalyanaraman, B., Rhee, S. G., Thornalley, P. J., Partridge, L., Gems, D., Nyström, T., Belousov, V., Schumacker, P. T. and Winterbourn, C. C. (2011). Unravelling the biological roles of reactive oxygen species. Cell Metabolism 13, 361366.
Nelson, D. P. and Kiesow, L. A. (1972). Enthalpy of the composition of hydrogen peroxide by catalase at 25 °C. Analytical Biochemistry 49, 474479.
Ohkawa, H., Ohishi, N. and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95, 351358.
Paglia, D. E. and Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocytes glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70, 158169.
Piedrafita, D., Spithill, T. W., Smith, R. E. and Raadsma, H. W. (2010). Improving animal and human health through understanding liver fluke immunology. Parasite Immunology 32, 572581.
Salimi-Bejestani, M. R., McGarry, J. W., Felstead, S., Ortiz, P., Akca, A. and Williams, D. J. L. (2005). Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test. Research in Veterinary Science 78, 177181.
Sarin, K., Kumar, A., Prakash, A. and Sharma, A. (1993). Oxidative stress and antioxidant defense mechanism in Plasmodium vivax malaria before and after chloroquine treatment. Indian Journal of Malariology 30, 127133.
Sena, L. A. and Chandel, N. S. (2012). Physiological roles of mitochondrial reactive oxygen species. Molecular and Cellular Biochemistry 48, 158167.
Shao, B., Zhu, L. S., Dong, M., Wang, J., Wang, J., Xie, H., Zhang, Q., Du, Z. and Zhu, S. (2012). DNA damage and oxidative stress induced by endosulfan exposure in zebrafish (Danio rerio). Ecotoxicology 21, 15331540.
Thrall, M. A., Weiser, G., Allison, R. W. and Campbell, T. W. (2012). Veterinary Hematology and Clinical Chemistry, 2nd edn. Wiley-Blackwell, State Avenue, Ames, Iowa. 762p.
Ueno, H. and Gonçalves, P. C. (1994). Manual for the Diagnosis of Helminths of Ruminants, 3nd edn. JICA, Porto Alegre, 62p.
Winterbourne, C. C. (2015). Are free radicals involved in thiol-based redox signaling? Free Radical Biology and Medicine 80, 164170.

Keywords

Oxidative stress and changes in adenosine deaminase activity of cattle experimentally infected by Fasciola hepatica

  • ALEKSANDRO S. DA SILVA (a1) (a2), MATHEUS D. BALDISSERA (a3), NATHIELI B. BOTTARI (a2), MATHEUS E. GABRIEL (a4), LEANDRO A. RHODEN (a4), MANOELA M. PIVA (a4), RICARDO CHRIST (a4), FERNANDA A. STEDILLE (a4), ANDERSON GRIS (a4), VERA M. MORSCH (a2), MARIA ROSA SCHETINGER (a2) and RICARDO E. MENDES (a4)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed