Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-05-10T14:20:59.274Z Has data issue: false hasContentIssue false
  • English
  • Français

The occurrence of antibodies to hidden and exposed determinants of surface antigens of Trichinella spiralis

Published online by Cambridge University Press:  06 April 2009

Guadalupe Ortega-Pierres ,
Ann Chayen ,
N. W. T. Clark  and
R. M. E. Parkhouse
Guadalupe Ortega-Pierres
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Ann Chayen
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
N. W. T. Clark
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
R. M. E. Parkhouse
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Summary

Mice were infected per os with Trichinella spiralis and their lymphocytes were removed and fused with mouse myeloma cell line P3 × 63Ag8653P3 for the selection of monoclonal antibodies to biochemically defined, stage-specific surface antigens of 3 parasite developmental stages: muscle larvae, adults and newborn larvae. Two separate antibodies against a defined single surface antigen of each stage were isolated. In each separate case the pair of monoclonal antibodies precipitated the same component from detergent-solubilized surface antigen preparations, but only one was able to bind to the surface of the living worm. The other must therefore be directed against an antigenic epitope which is obscured in the intact worm surface. The latter type of antibody is unlikely to be involved in the initial phase of parasite rejection and hence is another example of a non-protective host antibody response. The stimulus for its synthesis may be release of surface antigen, which does occur in vitro. One surface antigen of the newborn larvae is only detected by antibody in the first 6 h after birth; thereafter its presence is obscured as other antigens appear. The major surface antigen of the infective larvae contains carbohydrate determinants which are not available at the parasite surface. In addition, it displays great molecular heterogeneity but all variants appear to be derived from a common polypeptide structure.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 2 , April 1984 , pp. 359 - 369
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Clark, N. W. T., Philipp, M. & Parkeouse, R. M. E. (1982). Non-covalent interactions result in aggregation of surface antigens of the parasitic nematode Trichinella spiralis. The Biochemical Journal 206, 27–32.CrossRefGoogle ScholarPubMed
Dennis, D. T., Despommier, D. & Davis, N. (1970). Infectivity of the new born larvae of Trichinella spiralis in the rat. Journal of Parasitology 56, 974–7.CrossRefGoogle Scholar
Despommier, D. (1975). Adaptive changes in muscle fibres infected with Trichinella spiralis. American Journal of Pathology 78, 477–96.Google ScholarPubMed
Fasekas De St Groth, S. & Scheidegger, D. (1980). Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35, 1–21.CrossRefGoogle ScholarPubMed
Hunter, W. M. & Greenwood, F. C. (1962). Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194, 495–6.CrossRefGoogle ScholarPubMed
Jungery, M., Clark, N. W. T. & Parkhouse, R. M. E. (1983). A major change in surface antigens during the maturation of new born larvae of Trichinella spiralis. Molecular Biochemical Parasitology 7, 101–9.CrossRefGoogle Scholar
Kazura, J. W. & Aikawa, M. (1980). Host defence mechanisms against Trichinella spiralis infection in the mouse: eosinophil-mediated destruction of newborn larvae in vitro. Journal of immunology 124, 355–61.CrossRefGoogle ScholarPubMed
Kazura, J. W. & Grove, D. I. (1978). Stage-specific antibody-dependent eosinophil-mediated destruction of Trichinella spiralis. Nature, London 247, 588–9.CrossRefGoogle Scholar
Kearney, J. F., Radbrucu, A., Liesegang, B. & Rajewsky, K. (1979). A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody secreting hybrid cell lines. Journal of immunology 123, 1548–50.CrossRefGoogle ScholarPubMed
KÖhler, G. & Mllstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256, 495–7.CrossRefGoogle ScholarPubMed
Laskey, R. A. & Mills, A. D. (1977). Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitised films. FEBS Letters 82, 314–16.CrossRefGoogle Scholar
Mackenzie, C. D., Jungery, M., Taylor, P. M. & Ogilvie, B. M. (1980). Activation of complement, the induction of antibodies to the surface of nematodes and the effect of these factors and cells on worm survival in vitro. European Journal of immunology 10, 594–601.CrossRefGoogle Scholar
Mackenzie, C. D., Jungery, M., Taylor, P. M. & Ogilvie, B. M. (1981). The in vitro interaction of eosinophils, neutrophils, macrophages and mast cells with neinatode surfaces in the presence of complement or antibodies. Journal of Pathology 133, 161–75.CrossRefGoogle ScholarPubMed
Mackenzie, C. D., Preston, P. M. & Ogilvie, B. M. (1978). Immunological properties of the surface of parasitic nematodes. Nature, London 276, 826–8.CrossRefGoogle ScholarPubMed
Ogilvie, B. M., Philipp, M., Jungery, M., Maizels, R. M., Worms, M. J. & Parkhouse, R. M. E. (1980). The surface of nematodes and the immune response of the host. In The host—Invader interplay, (ed. van den Bossche, H.), pp. 90–5, Amsterdam, Elsevier North-Holland.Google Scholar
Parkhouse, R. M. E., Philipp, M. & Ogilvie, B. M. (1981). Characterisation of surface antigens of Trichinella spiralis infective larvae. Parasite immunology 3, 339–52.CrossRefGoogle ScholarPubMed
Philipp, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of parasitic nematodes. Nature, London 287, 538–40.CrossRefGoogle Scholar
Smithers, S. R. (1967). The induction and nature of antibody responses to parasites. In immunologic Aspects of Parasitic infections, no. 150, pp. 43–49. Pan American Health Organisation Scientific Publication.Google Scholar