Skip to main content Accessibility help

The occurrence of antibodies to hidden and exposed determinants of surface antigens of Trichinella spiralis

  • Guadalupe Ortega-Pierres (a1), Ann Chayen (a1), N. W. T. Clark (a1) and R. M. E. Parkhouse (a1)


Mice were infected per os with Trichinella spiralis and their lymphocytes were removed and fused with mouse myeloma cell line P3 × 63Ag8653P3 for the selection of monoclonal antibodies to biochemically defined, stage-specific surface antigens of 3 parasite developmental stages: muscle larvae, adults and newborn larvae. Two separate antibodies against a defined single surface antigen of each stage were isolated. In each separate case the pair of monoclonal antibodies precipitated the same component from detergent-solubilized surface antigen preparations, but only one was able to bind to the surface of the living worm. The other must therefore be directed against an antigenic epitope which is obscured in the intact worm surface. The latter type of antibody is unlikely to be involved in the initial phase of parasite rejection and hence is another example of a non-protective host antibody response. The stimulus for its synthesis may be release of surface antigen, which does occur in vitro. One surface antigen of the newborn larvae is only detected by antibody in the first 6 h after birth; thereafter its presence is obscured as other antigens appear. The major surface antigen of the infective larvae contains carbohydrate determinants which are not available at the parasite surface. In addition, it displays great molecular heterogeneity but all variants appear to be derived from a common polypeptide structure.



Hide All
Clark, N. W. T., Philipp, M. & Parkeouse, R. M. E. (1982). Non-covalent interactions result in aggregation of surface antigens of the parasitic nematode Trichinella spiralis. The Biochemical Journal 206, 2732.
Dennis, D. T., Despommier, D. & Davis, N. (1970). Infectivity of the new born larvae of Trichinella spiralis in the rat. Journal of Parasitology 56, 974–7.
Despommier, D. (1975). Adaptive changes in muscle fibres infected with Trichinella spiralis. American Journal of Pathology 78, 477–96.
Fasekas De St Groth, S. & Scheidegger, D. (1980). Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35, 121.
Hunter, W. M. & Greenwood, F. C. (1962). Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194, 495–6.
Jungery, M., Clark, N. W. T. & Parkhouse, R. M. E. (1983). A major change in surface antigens during the maturation of new born larvae of Trichinella spiralis. Molecular Biochemical Parasitology 7, 101–9.
Kazura, J. W. & Aikawa, M. (1980). Host defence mechanisms against Trichinella spiralis infection in the mouse: eosinophil-mediated destruction of newborn larvae in vitro. Journal of immunology 124, 355–61.
Kazura, J. W. & Grove, D. I. (1978). Stage-specific antibody-dependent eosinophil-mediated destruction of Trichinella spiralis. Nature, London 247, 588–9.
Kearney, J. F., Radbrucu, A., Liesegang, B. & Rajewsky, K. (1979). A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody secreting hybrid cell lines. Journal of immunology 123, 1548–50.
KÖhler, G. & Mllstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256, 495–7.
Laskey, R. A. & Mills, A. D. (1977). Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitised films. FEBS Letters 82, 314–16.
Mackenzie, C. D., Jungery, M., Taylor, P. M. & Ogilvie, B. M. (1980). Activation of complement, the induction of antibodies to the surface of nematodes and the effect of these factors and cells on worm survival in vitro. European Journal of immunology 10, 594601.
Mackenzie, C. D., Jungery, M., Taylor, P. M. & Ogilvie, B. M. (1981). The in vitro interaction of eosinophils, neutrophils, macrophages and mast cells with neinatode surfaces in the presence of complement or antibodies. Journal of Pathology 133, 161–75.
Mackenzie, C. D., Preston, P. M. & Ogilvie, B. M. (1978). Immunological properties of the surface of parasitic nematodes. Nature, London 276, 826–8.
Ogilvie, B. M., Philipp, M., Jungery, M., Maizels, R. M., Worms, M. J. & Parkhouse, R. M. E. (1980). The surface of nematodes and the immune response of the host. In The host—Invader interplay, (ed. van den Bossche, H.), pp. 90–5, Amsterdam, Elsevier North-Holland.
Parkhouse, R. M. E., Philipp, M. & Ogilvie, B. M. (1981). Characterisation of surface antigens of Trichinella spiralis infective larvae. Parasite immunology 3, 339–52.
Philipp, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of parasitic nematodes. Nature, London 287, 538–40.
Smithers, S. R. (1967). The induction and nature of antibody responses to parasites. In immunologic Aspects of Parasitic infections, no. 150, pp. 4349. Pan American Health Organisation Scientific Publication.


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed