Hostname: page-component-6d856f89d9-26vmc Total loading time: 0 Render date: 2024-07-16T08:21:32.102Z Has data issue: false hasContentIssue false
  • English
  • Français

Observations on tanning of the potato cyst-nematode, Globodera rostochiensis

Published online by Cambridge University Press:  06 April 2009

F. A. Awan  and
W. M. Hominick
F. A. Awan
Affiliation:
Department of Pure and Applied Biology, Imperial College at Silwood Park, Ashurst Lodge, Ascot, Berks.
W. M. Hominick
Affiliation:
Department of Pure and Applied Biology, Imperial College at Silwood Park, Ashurst Lodge, Ascot, Berks.
Get access Rights & Permissions [Opens in a new window]

Summary

Colour changes that occur during tanning of intact and punctured Globodera rostochiensis females and of isolated cuticle are described. Experiments with catechol and enzyme inhibitors show that the tanning enzyme, a phenoloxidase, is located in the cuticle, soluble in aqueous media and relatively abundant. Substrate for the enzyme is limited in the cuticle. Pseudocoelomic fluid of yellow females promoted tanning and contains either phenolic substrate(s) for the enzyme or part of a biochemical system responsible for release of the substrate. The substrate or biochemical system is thermolabile. Apparently, the tanning enzyme and its substrate(s) are partitioned in living females but the partitioning breaks down after death and the cuticle tans to form the cyst.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 1 , August 1982 , pp. 61 - 71
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Andersen, S. O. (1976). Cuticular enzymes and sclerotization in insects. In The Insect Integument (ed. Hepburn, H. R.), pp. 121–143. Amsterdam: Elsevier.Google Scholar
Clakke, A. J., Cox, P. M. & Shepherd, A. M. (1967). The chemical composition of the egg shells of the potato cyst-nematode, Heterodera rostochiensis Woll. The Biochemical Journal 104, 1056–60.Google Scholar
Clegg, J. A. & Smyth, J. D. (1968). Growth, development and culture methods: parasitic Platyhelminths. In Chemical Zoology vol. 2, (ed. Florkin, M. and Scheer, B. T.), pp. 395–466. London: Academic Press.CrossRefGoogle Scholar
Ellenby, C. (1946). Nature of the cyst wall of the potato-root eelworm Heterodera rostochiensis, Wollenweber, and its permeability to water. Nature 157, 302.CrossRefGoogle ScholarPubMed
Ellenby, C. (1963 a). Masked polyphenols in the cuticle of a cyst forming nematode. Experientia 19, 256.CrossRefGoogle Scholar
Ellenby, C. (1963 b). The gold of the golden nematode of potatoes. Proceedings of the 16th International Congress of Zoology 1, 139.Google Scholar
Evans, K. & Stone, A. R. (1977). A review of the distribution and biology of the potato cyst-nematodes Globodera rostochiensis and G. pallida. Pest Articles and News Summaries 23, 178–89.Google Scholar
Guile, C. T. (1966). Cyst chromogenesis in potato cyst eelworm pathotypes. Plant Pathology 15, 125–8.CrossRefGoogle Scholar
Guile, C. T. (1967). On cyst colour changes, bionomics and distribution of potato cyst-eelworm (Heterodera rostochiensis Woll.) pathotypes in the East Midlands. Annals of Applied Biology 60, 411–19.CrossRefGoogle Scholar
Hominick, W. M. (1979). Selection for hatching at low temperatures in Globodera rostochiensis by continuous cultivation of early potatoes. Nematologica 25, 322–32.CrossRefGoogle Scholar
Neville, A. C. (1975). Biology of the Arthropod Cuticle. Berlin: Springer-Verlag.CrossRefGoogle Scholar
Pryor, M. G. M. (1962). Sclerotization. In Comparative Biochemistry, vol. 4B, (ed. Florkin, M. and Mason, H. S.), pp. 371–396. London: Academic Press.CrossRefGoogle Scholar
Shepherd, A. M. & Clark, S. A. (1978). Cuticle structure and ‘cement’formation at the anterior end of female cyst-nematodes of the genera Heterodera and Globodera (Heteroderidae: Tylenchida). Nematologica 24, 201–8.Google Scholar
Smith, L. & Ellenby, C. (1967). Some biochemical changes in the contents of the cyst of Heterodera rostochiensis during its maturation. Nematologica 13, 395–405.CrossRefGoogle Scholar
Vincent, J. F. V. & Hillerton, J. E. (1979). The tanning of insect cuticle – a critical review and a revised mechanism. Journal of Insect Physiology 25, 653–8.CrossRefGoogle Scholar
Wharton, D. (1980). Nematode egg-shells. Parasitology 81, 447–63.CrossRefGoogle ScholarPubMed
Wilson, E. M. (1971). The changes in cyst form during the development of the cyst of Heterodera rostochiensis and H. schachtii. Journal of Helminthology 45, 211–22.CrossRefGoogle Scholar