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A novel miRNA, miR-13664, targets CpCYP314A1 to regulate deltamethrin resistance in Culex pipiens pallens

  • X. H. Sun (a1) (a2), N. Xu (a1) (a2), Y. Xu (a1) (a2), D. Zhou (a1) (a2), Y. Sun (a1) (a2), W. J. Wang (a3), L. Ma (a1) (a2), C. L. Zhu (a1) (a2) and B. Shen (a1) (a2)...


Extensive insecticide use has led to the resistance of mosquitoes to these insecticides, posing a major barrier to mosquito control. Previous Solexa high-throughput sequencing of Culex pipiens pallens in the laboratory has revealed that the abundance of a novel microRNA (miRNA), miR-13664, was higher in a deltamethrin-sensitive (DS) strain than a deltamethrin-resistant (DR) strain. Real-time quantitative PCR revealed that the miR-13664 transcript level was lower in the DR strain than in the DS strain. MiR-13664 oversupply in the DR strain increased the susceptibility of these mosquitoes to deltamethrin, whereas inhibition of miR-13664 made the DS strain more resistant to deltamethrin. Results of bioinformatic analysis, quantitative reverse-transcriptase polymerase chain reaction, luciferase assay and miR mimic/inhibitor microinjection revealed CpCYP314A1 to be a target of miR-13664. In addition, downregulation of CpCYP314A1 expression in the DR strain reduced the resistance of mosquitoes to deltamethrin. Taken together, our results indicate that miR-13664 could regulate deltamethrin resistance by interacting with CpCYP314A1, providing new insights into mosquito resistance mechanisms.


Corresponding author

Author for correspondence: B. Shen, E-mail: and C.L. Zhu,


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A novel miRNA, miR-13664, targets CpCYP314A1 to regulate deltamethrin resistance in Culex pipiens pallens

  • X. H. Sun (a1) (a2), N. Xu (a1) (a2), Y. Xu (a1) (a2), D. Zhou (a1) (a2), Y. Sun (a1) (a2), W. J. Wang (a3), L. Ma (a1) (a2), C. L. Zhu (a1) (a2) and B. Shen (a1) (a2)...


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