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Murine hybridoma-derived antibodies in the processing of antigens for the immunodiagnosis of hydatid (Echinococcus granulosus) infection in sheep

Published online by Cambridge University Press:  08 November 2011

P. S. Craig ,
R. E. Hocking ,
G. F. Mitchell  and
M. D. Rickard
P. S. Craig
Affiliation:
Veterinary Clinical Centre, University of Melbourne, Princes Highway, Werribee, Victoria 3030, Australia
R. E. Hocking
Affiliation:
Laboratory of Immunoparasitology, The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Victoria, Australia
G. F. Mitchell
Affiliation:
Veterinary Clinical Centre, University of Melbourne, Princes Highway, Werribee, Victoria 3030, Australia
M. D. Rickard
Affiliation:
Veterinary Clinical Centre, University of Melbourne, Princes Highway, Werribee, Victoria 3030, Australia
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Summary

Mice experimentally infected with the hydatid parasite Echinococcus granulosus (secondary echinococcosis), were used as spleen cell donors for hybridoma production. Two hybridomas were produced which secreted antibody with anti-E. granulosus cyst fluid (EgCF) activity. Radio-immunoassays comparing the binding of these hybridoma-derived antibodies to E. granulosus versus several other antigen preparations from sheep parasites showed that the antibodies had a high degree of specificity for the hydatid parasite. However, using a panel of clinically defined sheep sera in a competitive radio-immunoassay, the binding of the hybridoma-derived antibodies to EgCF was inhibited strongly by sera from sheep infected with the common parasites Fasciola hepatica and Taenia hydatigena. When the hybridoma-derived antibodies were conjugated to CNBr-activated Sepharose and the crude EgCF applied to the affinity column, the ‘run through’ antigen preparation showed a significant increase in specificity for experimental E. granulosus infection. A useful means of applying the hybridoma technology in the development of new immunodiagnostic reagents may be to select those murine hybridomas which produce high-affinity, cross-reacting antibodies and to use these antibodies in the processing of homologous antigen by affinity chromatography.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 2 , October 1981 , pp. 303 - 317
Copyright
Copyright © Cambridge University Press 1981

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