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Morphometric, genetic diversity and phylogenetic analysis of Taenia hydatigena (Pallas, 1766) larval stage in Iranian livestock

Published online by Cambridge University Press:  15 October 2019

Shahabeddin Sarvi ,
Laya Ebrahimi Behrestaghi ,
Abbas Alizadeh ,
Seyed Abdollah Hosseini ,
Shaban Gohardieh ,
Reza Bastani ,
Jamshid Yazdani Charati ,
Ahmad Daryani ,
Afsaneh Amouei  and
Adel Spotin Open the ORCID record for Adel Spotin [Opens in a new window]
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Shahabeddin Sarvi
Affiliation:
Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Laya Ebrahimi Behrestaghi
Affiliation:
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
Abbas Alizadeh
Affiliation:
Mazandaran Provincial Veterinary Service, Sari, Iran
Seyed Abdollah Hosseini
Affiliation:
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Shaban Gohardieh
Affiliation:
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Reza Bastani
Affiliation:
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
Jamshid Yazdani Charati
Affiliation:
Department of Biostatistics, Faculty of Health, Mazandarn University of Medical Science, Sari, Iran
Ahmad Daryani
Affiliation:
Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Afsaneh Amouei
Affiliation:
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Adel Spotin*
Affiliation:
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
Shirzad Gholami*
Affiliation:
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
*
Author for correspondence: Adel Spotin, E-mail: adelespotin@gmail.com, Spotina@tbzmed.ac.ir and Shirzad Gholami, E-mail: sgholami200@gmail.com
Author for correspondence: Adel Spotin, E-mail: adelespotin@gmail.com, Spotina@tbzmed.ac.ir and Shirzad Gholami, E-mail: sgholami200@gmail.com
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Abstract

Cysticercus tenuicollis as metacestode of Taenia hydatigena is the most prevalent taeniid species in livestock. Eighty-eight C. tenuicollis samples were collected from sheep (n = 44) and goats (n = 44) of the northern Iran from 2015 to 2016. The isolated parasites were characterized by morphometric keys. The DNA of the larval stage was extracted, amplified and sequenced targeting mitochondrial 12S rRNA and Cox 1 markers. A significant difference in larval rostellar hook length was observed in 12S rRNA haplotypes. Analysis of molecular variance of 12S rRNA indicated a moderate genetic diversity in the C. tenuicollis isolates. The pairwise sequence distance of C. tenuicollis showed an intra-species diversity of 0.3–0.5% and identity of 99.5–100%. Using the 12S rRNA sequence data we found a moderate genetic difference (Fst; 0.05421) in C. tenucollis isolates collected from livestock of the northern and southeastern regions of Iran. We concluded that the genetic variants of C. tenuicollis are being undoubtedly distributing mostly in different parts of Iran. Further studies with a larger number of T. hydatigena isolates collected from various intermediate and definitive hosts are needed to study this evolutionary assumption and also to determine the apparent genetic differences observed in the studied regions.

Keywords

Cysticercus tenuicollislivestockmitochondrial DNAnorthern IranTaenia hydatigena
Type
Research Article
Information
Parasitology , Volume 147 , Issue 2 , February 2020 , pp. 231 - 239
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Copyright
Copyright © Cambridge University Press 2019

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Footnotes

*

Contributed equally.

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