Skip to main content Accessibility help
×
Home

Molecular phylogenetic analysis of the genus Gyrodactylus (Platyhelminthes: Monogenea) inferred from rDNA ITS region: subgenera versus species groups

  • I. MATEJUSOVÁ (a1), M. GELNAR (a2), O. VERNEAU (a3), C. O. CUNNINGHAM (a1) and D. T. J. LITTLEWOOD (a4)...

Abstract

Analyses of small subunit ribosomal RNA gene sequences of representatives of major taxa of Monopisthocotylea were performed to identify the sister group of Gyrodactylus. Nuclear ribosomal DNA sequencesNucleotide sequence data reported in this paper are available in the GenBank, DDBJ and EMBL databases under the accession numbers AJ566375-79, AJ566768, AJ567670-74, AJ581657. from the complete internal transcribed spacer (ITS) region were used to infer phylogeny of 37 Gyrodactylus species and Gyrodactyloides bychowskii, Macrogyrodactylus polypteri and Gyrdicotylus gallieni, using maximum likelihood, parsimony and Bayesian inference. The genus Gyrodactylus appeared to be a monophyletic group in all analyses, based on the present data set. Within the genus, there were 3 major groups recognized by high bootstrap values and posterior probabilities. None of the 6 subgenera appeared to be monophyletic, and the most basal subgenus G. (Gyrodactylus) was paraphyletic. Characteristics of the excretory system of Gyrodactylus do not seem to be conservative enough to reveal subgenera within Gyrodactylus and we suggest abandoning existing subgenera as indicators of phylogeny. The grouping of species based on the morphology of the ventral bar and marginal hooks seems to have sufficient power to infer relationships between the Gyrodactylus species.

Copyright

Corresponding author

FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, UK. Tel: +44 1224 295 683. Fax: +44 1224 295 667. E-mail: I.Matejusova@marlab.ac.uk

References

Hide All

REFERENCES

AUDEBERT, F., DURETTE-DESSET, M.-C. & CHILTON, N. B. ( 2000). Internal transcribed spacer rDNA can be used to infer the phylogenetic relationships of species within the genus Nematodirus (Nematoda: Molineoidea). International Journal for Parasitology 30, 187191.
BAKKE, T. A., HARRIS, P. D. & CABLE, J. ( 2002). Host specificity dynamics: observations on gyrodactylid monogeneans. International Journal for Parasitology 32, 281308.
BENTZ, S., LEROY, S., DUPREEZ, L., MARIAUX, J., VAUCHER, C. & VERNEAU, O. ( 2001). Origin and evolution of African Polystoma (Monogenea: Polystomatidae) assessed by molecular methods. International Journal for Parasitology 31, 697705.
CABLE, J., HARRIS, P. D., TINSLEY, R. C. & LAZARUS, C. M. ( 1999). Phylogenetic analysis of Gyrodactylus spp. (Platyhelminthes: Monogenea) using ribosomal DNA sequences. Canadian Journal of Zoology 77, 14391449.
CUNNINGHAM, C. O. ( 1997). Species variation within the internal transcribed spacer (ITS) region of Gyrodactylus (Monogenea: Gyrodactylidae) ribosomal RNA genes. Journal of Parasitology 83, 215219.
CUNNINGHAM, C. O., McGILLIVRAY, D. M., MACKENZIE, K. & MELVIN, W. T. ( 1995 a). Discrimination between Gyrodactylus salaris, G. derjavini and G. truttae (Platyhelminthes: Monogenea) using restriction fragment length polymorphisms and an oligonucleotide probe within the small subunit ribosomal RNA gene. Parasitology 111, 8794.
CUNNINGHAM, C. O., McGILLIVRAY, D. M. & MACKENZIE, K. ( 1995 b). Phylogenetic analysis of Gyrodactylus salaris Malmberg, 1957 based on the small subunit (18S) ribosomal RNA gene. Molecular and Biochemical Parasitology 71, 139142.
CUNNINGHAM, C. O., MO, T. A., COLLINS, C. M., BUCHMANN, K., THIERY, R., BLANC, G. & LAUTRAITE, A. ( 2001). Redescription of Gyrodactylus teuchis Lautraite, Blanc, Thiery, Daniel & Vigneulle, 1999 (Monogenea: Gyrodactylidae), a species identified by ribosomal RNA sequence. Systematic Parasitology 48, 141150.
ERGENS, R. ( 1966). Revision of the helminthofauna of fishes from Czechoslovakia IV. Folia Parasitologica, 13, 212221.
ERGENS, R. ( 1976). Gyrodactylus barbi sp. n. (Monogenoidea) from the fins of barbels. Vestnik Ceskoslovenske Spolecnosti Zoologicke 40, 19611962.
ERGENS, R. ( 1980). On the problem of three species of the genus Gyrodactylus, members of “G. wageneri-group” (Gyrodactylidae: Monogenea). Helminthologia 17, 257267.
ERGENS, R. & BYCHOWSKY, B. E. ( 1967). Revision of the species Gyrodactylus nemachili Bychowsky, 1936 (Monogenoidea). Folia Parasitologica 14, 225238.
ERGENS, R. & YUKHIMENKO, S. S. ( 1975). Gyrodactylus (Monogenoidea) from some Rhodeinae (Cypriniformes). Folia Parasitologica 22, 3336.
ERNST, I., FLETCHER, A. & HAYWARD, C. ( 2000). Gyrodactylus anguillae (Monogenea: Gyrodactylidae) from anguilid eels (Anguilla australis and Anguilla reinhardtii) in Australia: a native or an exotic? Journal of Parasitology 86, 11521156.
FARRIS, J. S., KÄLLERSJO, M., KLUGE, A. G. & BULT, C. ( 1994). Testing significance of incongruence. Cladistics 10, 315319.
GLÄSER, H.-J. ( 1974 a). Sechs neue Arten der Gyrodactylus-wageneri Gruppe (Monogenea, Gyrodactylidae) nebst Bemerkungen zur Preparation, Determination, Terminologie und Wirtsspezifität. Zoologischer Anzeiger 192, 5676.
GLÄSER, H.-J. ( 1974 b). Eine neue Artengruppe des Subgenus Gyrodactylus (Paranephrotus) (Monogenea, Gyrodactylidae). Zoologischer Anzeiger 192, 271278.
HARRIS, P. D. ( 1985). Species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae) from freshwater fishes in southern England, with a description of Gyrodactylus rogatensis sp. Nov. from the bullhead Cottus gobio L. Journal of Natural History 19, 791809.
HARRIS, P. D. ( 1986). Species of Gyrodactylus von Nordmann, 1832 (Monogenea, Gyrodactylidae) from poeciliid fishes, with a description of G. turnbulli sp., nov. from the guppy, Poecilia reticulata Peters. Journal of Natural History 20, 183191.
HARRIS, P. D. & CABLE, J. ( 2000). Gyrodactylus poeciliae n. sp. and G. milleri n. sp. (Monogenea: Gyrodactylidae) from Poecilia caucana (Steindachner) in Venezuela. Systematic Parasitology 47, 7985.
HUELSENBECK, J. P. & RONQUIST, F. ( 2001). MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754755.
HUELSENBECK, J. P., RONQUIST, F., NIELSEN, R. & BOLLBACK, J. P. ( 2001). Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294, 23102314.
HUYSE, T. & VOLCKAERT, F. A. M. ( 2002). Identification of a host-associated species complex using molecular and morphometric analyses, with the description of Gyrodactylus rugiensoides n. sp. (Gyrodactylidae, Monogenea). International Journal for Parasitology 32, 907919.
JEANMOUGIN, F., THOMPSON, J. D., GOUY, M., HIGGINS, D. G. & GIBSON, T. J. ( 1998). Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences 23, 403405.
MADDISON, W. P. & MADDISON, D. R. ( 2000). MacClade. Version 4. Sinauer Associates, Sunderland, Massachusetts.
MALMBERG, G. ( 1956). Om förekomsten av Gyrodactylus på svenska fiskar. Skrifter utgivna av Södra Sveriges Fiskeriförening, Årsskrift, pp. 1976.
MALMBERG, G. ( 1964). Taxonomical and ecological problems in Gyrodactylus (Trematoda, Monogenea). In Parasitic Worms and Aquatic Conditions ( ed. Ergens, R. & Rysavy, B. ), pp. 203230. Czech Academy of Science, Prague, Czech Republic.
MALMBERG, G. ( 1970). The excretory systems and the marginal hooks as a basis for the systematics of Gyrodactylus (Trematoda, Monogenea). Arkiv för Zoologi, Serie 2, Band, 23 (1). Almqvist & Wiksell, Stockholm.
MALMBERG, G. ( 1998). On the evolution within the family Gyrodactylidae (Monogenea). International Journal for Parasitology 28, 16251635.
MATEJUSOVA, I., GELNAR, M., McBEATH, A. J. A., COLLINS, C. M. & CUNNINGHAM, C. O. ( 2001). Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). International Journal for Parasitology 31, 738745.
OLSON, P. D. & LITTLEWOOD, D. T. J. ( 2002). Phylogenetics of the Monogenea – evidence from a medley of molecules. International Journal for Parasitology 32, 233244.
POSADA, D. & CRANDALL, K. A. ( 1998). Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817818.
STEANE, D. A., McKINNON, G. E., VAILLANCOURT, R. E. & POTTS, B. M. ( 1999). ITS sequence data resolve higher level relationships among the eucalypts. Molecular Phylogenetics and Evolution 12, 215223.
SWOFFORD, D. L. ( 2002). PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). 4th Edn. Sinauer Associates, Sunderland, Massachusetts.
WILLIAMS, H. & JONES, A. ( 1994). Parasitic worms of fish. Taylor and Francis, London.
ZIETARA, M. S., ARNDT, A., GEETS, A., HELLEMANS, B. & VOLCKAERT, F. A. M. ( 2000). The nuclear rDNA region of Gyrodactylus arcuatus and G. branchicus (Monogenea: Gyrodactylidae). Journal of Parasitology 86, 13681373.
ZIETARA, M. S., HUYSE, T., LUMME, J. & VOLCKAERT, F. A. M. ( 2002). Deep divergence among subgenera of Gyrodactylus inferred from rDNA ITS region. Parasitology 124, 3952.
ZIETARA, M. S. & LUMME, J. ( 2002). Speciation by host switch and adaptive radiation in a fish parasite genus Gyrodactylus (Monogenea, Gyrodactylidae). Evolution 56, 24452458.
ZITNAN, R. ( 1964). Gyrodactylus rhodei sp. n. – a new monogenean species from skin of Rhodeus sericeus amarus (Bloch). Helminthologia 5 , 14.

Keywords

Molecular phylogenetic analysis of the genus Gyrodactylus (Platyhelminthes: Monogenea) inferred from rDNA ITS region: subgenera versus species groups

  • I. MATEJUSOVÁ (a1), M. GELNAR (a2), O. VERNEAU (a3), C. O. CUNNINGHAM (a1) and D. T. J. LITTLEWOOD (a4)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed