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Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica

  • K. CHAITHIRAYANON (a1), R. GRAMS (a2), S. VICHASRI-GRAMS (a3), A. HOFMANN (a4), G. KORGE (a4), V. VIYANANT (a2), E. S. UPATHAM (a3) and P. SOBHON (a1)...

Abstract

A cDNA encoding Fg14-3-3 protein 1 was cloned by immunoscreening of an adult-stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental antigens of the parasite. The protein has a deduced amino acid sequence of 252 residues and a calculated molecular weight of 28·7 kDa. It shows sequence identity values between 57·6 and 58·1% to the human 14-3-3 beta, zeta, theta, and eta proteins and is in a phylogenetic cluster with the 14-3-3 protein 1 of Schistosoma spp. Nucleic acid analyses indicate that the Fg14-3-3 protein 1 is encoded by a single copy gene and that this gene is expressed as a transcript of 1250 nucleotides. In adult and 4-week-old parasites the gene's transcriptional and translational products were localized in the gut epithelium, parenchyma, tegument cells, and in the reproductive organs. An antiserum against recombinant Fg14-3-3 protein 1 detected a slightly smaller 14-3-3 protein in the parasite's excretion/secretion material and showed cross-reactivity with 14-3-3 proteins in extracts of other trematodes and mouse. Antibodies against Fg14-3-3 protein were detected in the sera of rabbits as early as 2 weeks after infection with metacercariae of F. gigantica and the antibody titre increased continuously over a 10-week observation period.

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Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok 10400, Thailand. Tel: +662 201 5418. Fax: +662 354 7168. E-mail: scpso@mucc.mahidol.ac.th

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Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica

  • K. CHAITHIRAYANON (a1), R. GRAMS (a2), S. VICHASRI-GRAMS (a3), A. HOFMANN (a4), G. KORGE (a4), V. VIYANANT (a2), E. S. UPATHAM (a3) and P. SOBHON (a1)...

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