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A middle-repetitive DNA sequence element in the sheep parasitic nematode, Trichostrongylus colubriformis

Published online by Cambridge University Press:  06 April 2009

M. J. Callaghan  and
K. J. Beh
M. J. Callaghan
Affiliation:
CSIRO Division of Animal Health, McMaster Laboratory, Private Bag #1, P.O. Glebe, NSW 2037, Australia
K. J. Beh
Affiliation:
CSIRO Division of Animal Health, McMaster Laboratory, Private Bag #1, P.O. Glebe, NSW 2037, Australia
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Summary

A novel repetitive DNA sequence in the sheep parasitic nematode Trichostrongylus colubriformis was cloned and sequenced. A l·1 kb repetitive sequence (Tc15) which hybridized with DNA from T. colubriformis but not with DNA from two other parasitic nematodes, Haemonchus contortus and Ostertagia circumcincta, or sheep was further characterized. Southern blot analysis showed that the repeat hybridized to a range of fragments in restriction digested T. colubriformis DNA and existed in multiple copy number tandem arrays. However, to define clearly the repetitive monomeric unit further screening of phagemid libraries containing BamH I restriction fragments using a subclone of Tc15 as a probe was carried out. Restriction map and sequence data were compiled for 3 clones containing a 145 bp highly repetitive sequence (designated TcREP) which shared homology with the original pTc15 clone. TcREP hybridized to a tandemly repeating sequence monomer of 145 bp in T. colubriformis DNA which was cloned from various genetic environments in the T. colubriformis genome. TcREP homologous sequences were also found in the genomes of two other species of the same genus (Trichostrongylus axei and Trichostrongylus vitrinus) but not in a fourth species (Trichostrongylus rugatus).

Keywords

tandem repeatsspecies-specific repeatscopy number
Type
Research Article
Information
Parasitology , Volume 109 , Issue 3 , September 1994 , pp. 345 - 350
Copyright
Copyright © Cambridge University Press 1994

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