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Microfluidic bioassay to characterize parasitic nematode phenotype and anthelmintic resistance

Published online by Cambridge University Press:  21 July 2010

BAOZHEN CHEN ,
ALEX DEUTMEYER ,
JOHN CARR ,
ALAN P. ROBERTSON ,
RICHARD J. MARTIN  and
SANTOSH PANDEY
BAOZHEN CHEN
Affiliation:
Department of Electrical and Computer Engineering, Iowa State University, Ames, IA 50011, USA
ALEX DEUTMEYER
Affiliation:
Department of Electrical and Computer Engineering, Iowa State University, Ames, IA 50011, USA
JOHN CARR
Affiliation:
Department of Electrical and Computer Engineering, Iowa State University, Ames, IA 50011, USA
ALAN P. ROBERTSON
Affiliation:
Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, USA
RICHARD J. MARTIN*
Affiliation:
Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, USA
SANTOSH PANDEY
Affiliation:
Department of Electrical and Computer Engineering, Iowa State University, Ames, IA 50011, USA
*
*Corresponding author: Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, USA. Tel: +515 294 2470. Fax: +515 294 3637. E-mail: rjmartin@iastate.edu
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Summary

With increasing resistance to anti-parasitic drugs, it has become more important to detect and recognize phenotypes of resistant isolates. Molecular methods of detecting resistant isolates are limited at present. Here, we introduce a microfluidic bioassay to measure phenotype using parameters of nematode locomotion. We illustrate the technique on larvae of an animal parasite Oesophagostomum dentatum. Parameters of sinusoidal motion such as propagation velocity, wavelength, wave amplitude, and oscillation frequency depended on the levamisole-sensitivity of the isolate of parasitic nematode. The levamisole-sensitive isolate (SENS) had a mean wave amplitude of 135 μm, which was larger than 123 μm of the levamisole-resistant isolate (LEVR). SENS had a mean wavelength of 373 μm, which was less than 393 μm of LEVR. The mean propagation velocity of SENS, 149 μm s−1, was similar to LEVR, 143 μm s−1. The propagation velocity of the isolates was inhibited by levamisole in a concentration-dependent manner above 0·5 μm. The EC50 for SENS was 3 μm and the EC50 for LEVR was 10 μm. This microfluidic technology advances present-day nematode migration assays and provides a better quantification and increased drug sensitivity. It is anticipated that the bioassay will facilitate study of resistance to other anthelmintic drugs that affect locomotion.

Keywords

microfluidicsbioassayOesophagostomum dentatumlevamisole resistancephenotype
Type
Research Article
Information
Parasitology , Volume 138 , Issue 1 , January 2011 , pp. 80 - 88
Copyright
Copyright © Cambridge University Press 2010

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