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Membrane internalization processes in different stages of Schistosoma mansoni as shown by a styryl dye (Frei Mao 1–43)

  • F. RIBEIRO (a1), P. M. Z. COELHO (a1) (a2), L. Q. VIEIRA (a1) (a3), K. POWELL (a1) and J. R. KUSEL (a1)...

Abstract

We have used a styryl dye FM 1–43 that cannot cross membranes passively to evaluate membrane vesicle internalization processes from the heptalaminate surface membrane in schistosomula and adult Schistosoma mansoni. The process of dye internalization was faster in adult worms than in 3 h skin or 24-h-old mechanically transformed schistosomula. Dye internalization and vesicle formation were inhibited at low temperature which suggests that the process is energy dependent. Dye internalization was observed to be a result of vesicle formation. This process is impaired by primaquine independently of the parasite stage. The specific Ca2+ chelator EGTA affected dye internalization at a concentration of 5 mm. When parasites were labelled in the presence of genistein (a tyrosine kinase inhibitor) uptake of the dye was blocked in 24-h-old schistosomula. However, only a partial blockage of the dye internalization was seen in adult worms. These results suggest that dye internalization mediated by tyrosine kinase signalling is developmentally regulated.

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Corresponding author: Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, The University of Glasgow, Glasgow G12 8QQ, UK. Tel: +44 141 330 6449. Fax: +44 141 330 4620. E-mail: gbca66@ucdf.gla.ac.uk.

Keywords

Membrane internalization processes in different stages of Schistosoma mansoni as shown by a styryl dye (Frei Mao 1–43)

  • F. RIBEIRO (a1), P. M. Z. COELHO (a1) (a2), L. Q. VIEIRA (a1) (a3), K. POWELL (a1) and J. R. KUSEL (a1)...

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