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Mechanisms underlying reduced expulsion of a murine nematode infection during protein deficiency

Published online by Cambridge University Press:  02 October 2007

T. TU ,
K. G. KOSKI  and
M. E. SCOTT
T. TU
Affiliation:
School of Dietetics and Human Nutrition, McGill University (Macdonald Campus) 21, 111 Lakeshore Road, Ste-Anne de Bellevue, QC H9X 3V9, Canada
K. G. KOSKI
Affiliation:
School of Dietetics and Human Nutrition, McGill University (Macdonald Campus) 21, 111 Lakeshore Road, Ste-Anne de Bellevue, QC H9X 3V9, Canada
M. E. SCOTT*
Affiliation:
Institute of Parasitology, McGill University (Macdonald Campus) 21, 111 Lakeshore Road, Ste-Anne de Bellevue, QC H9X 3V9, Canada
*
*Corresponding author: Institute of Parasitology, McGill University (Macdonald Campus), 21, 111 Lakeshore Road, Ste-Anne de Bellevue, QC H9X 3V9, Canada. Tel: +514 398 7996. Fax: +514 398 7857. E-mail: marilyn.scott@mcgill.ca
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Summary

Balb/c mice infected with the gastrointestinal nematode Heligmosomoides bakeri were fed protein sufficient (PS, 24%) or deficient (PD, 3%) diets to investigate whether diet, infection or dose of larval challenge (0, 100 or 200 larvae) influenced gut pathophysiology and inflammation. Among the PS mice, worms were more posteriorad in the intestine of mice infected with 200 compared with 100 larvae, suggesting active expulsion in the more heavily infected mice. This was consistent with the positive correlation between worm numbers and fluid leakage in PS mice; similar patterns were not detected in the PD mice. Infection also induced villus atrophy, which was more pronounced in PS than in PD mice. Our cytokine screening array indicated that infection in PD mice elevated a wide range of pro-inflammatory cytokines and chemokines. Whereas serum leptin concentrations were higher in PD mice, monocyte chemotactic protein-5 (MCP-5) in serum increased with increasing larval dose and concentrations were lower in PD than PS mice. We suggest that elevated MCP-5 together with villus atrophy may contribute to the apparent dose-dependent expulsion of H. bakeri from PS mice but that delayed expulsion in PD mice appeared related to a predominant Th1 cytokine profile that may be driven by leptin.

Keywords

Heligmosomoides bakerinematodeprotein deficiencyleptinvillus atrophyMCP-5histopathology
Type
Research Article
Information
Parasitology , Volume 135 , Issue 1 , January 2008 , pp. 81 - 93
Copyright
Copyright © Cambridge University Press 2007

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