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Liposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi

Published online by Cambridge University Press:  28 January 2014

CAMILA BELMONTE OLIVEIRA ,
LUCAS ALMEIDA RIGO ,
LUCIANA DALLA ROSA ,
LUCAS TREVISAN GRESSLER ,
CARINE ELOISE PRESTES ZIMMERMANN ,
ALINE FERREIRA OURIQUE ,
ALEKSANDRO SCHAFER DA SILVA ,
LUIZ C. MILETTI ,
RUY CARLOS RUVER BECK  and
SILVIA GONZALEZ MONTEIRO
CAMILA BELMONTE OLIVEIRA*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
LUCAS ALMEIDA RIGO
Affiliation:
Department of Production and Control of Medicines, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Brazil
LUCIANA DALLA ROSA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
LUCAS TREVISAN GRESSLER
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
CARINE ELOISE PRESTES ZIMMERMANN
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
ALINE FERREIRA OURIQUE
Affiliation:
Department of Production and Control of Medicines, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Brazil
ALEKSANDRO SCHAFER DA SILVA
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina – UDESC, Brazil
LUIZ C. MILETTI
Affiliation:
Department of Animal Production, Universidade do Estado de Santa Catarina, Lages, SC, Brazil
RUY CARLOS RUVER BECK
Affiliation:
Department of Production and Control of Medicines, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Brazil
SILVIA GONZALEZ MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
*
*Corresponding author: Departamento de Microbiologia e Parasitologia da UFSM, Faixa de Camobi – Km 9, Campus Universitário, Santa Maria, RS, Brasil. E-mail: camilabelmontevet@yahoo.com.br
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Summary

This study aimed to develop and test the in vitro and in vivo effectiveness of diminazene aceturate encapsulated into liposomes (L-DMZ) on Trypanosoma evansi. To validate the in vitro tests with L-DMZ, the efficacy of a commercial formulation of diminazene aceturate (C-DMZ) was also assessed. The tests were carried out in culture medium for T. evansi, at concentrations of 0·25, 0·5, 1, 2 and 3 μg mL−1 of L-DMZ and C-DMZ. A dose-dependent effect was observed for both formulations (L-DMZ and C-DMZ), with the highest dose-dependent mortality of trypomastigotes being observed at 1 and 3 h after the onset of tests with L-DMZ. The results of in vivo tests showed the same effects in the animals treated with L-DMZ and C-DMZ in single doses of 3·5 mg kg−1 and for 5 consecutive days (3·5 mg kg−1 day−1). It was possible to conclude that T. evansi showed greater in vitro susceptibility to L-DMZ when compared with C-DMZ. In vivo tests suggest that treatment with the L-DMZ and C-DMZ showed similar efficacy in vivo. The potential of the formulation developed in this study was clearly demonstrated, as it increased the efficacy of the treatment against trypanosomosis, but more studies are needed to increase the effectiveness in vivo.

Keywords

Nanotechnologydiamidinestrypanosomosis
Type
Research Article
Information
Parasitology , Volume 141 , Issue 6 , May 2014 , pp. 761 - 769
Copyright
Copyright © Cambridge University Press 2014 

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