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Killing of schistosomula of Schistosoma mansoni by macrophages: induction by T-cell clone-derived lymphokines and interferon-gamma

Published online by Cambridge University Press:  06 April 2009

C. F. Kubelka ,
A. Ruppel ,
P. H. Krammer  and
D. Gemsa
C. F. Kubelka
Affiliation:
Institute for Immunology and Serology, University of Heidelberg, F.KG.
A. Ruppel
Affiliation:
Institute for Immunology and Serology, University of Heidelberg, F.KG.
P. H. Krammer
Affiliation:
Institute for Immunology and Genetics of the German Cancer Research Center, Heidelberg, F.R.G.
D. Gemsa
Affiliation:
Institute for Immunology and Serology, University of Heidelberg, F.KG.
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Summary

The induction of schistosomulicidal activity of peritoneal macrophages by concanavalin A-stimulated supernatants from long-term T-cell clones and by interferon-gamma (IFN-γ) was investigated in detail. Optimal conditions of in vitro macrophage activation by T-cell clone supernatants were established. Macrophages from 13-week S. mansoni-infected mice responded to lymphokine activation as well as resident mnacrophages from uninfecteci mice. IFN-γ was shown to play an essential role in induction of schistosomulicidal macrophage activity: recombinant IFN-γ at high concentration could induce schistosomula killing, and an anti-IFN-γ antiserum inhibited the induction ofschistosomulicidal activity by T-cell clone supernatants. Our data also indicate that macrophage activation could be obtained by IFN-γ in synergy with other lymphokines in the supernatant of long-term T-cell clones. Macrophages from mice injected with T-cell clone supernatants were primed in vivo and triggered to kill schistosomula in vitro in the presence of lipopolysaccharide (LPS). The data demonstrate that lymphokines produced by T-cell clones and, in particular, IFN-γ can participate in the activation of schistosomulicidal macrophages.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 2 , April 1986 , pp. 325 - 336
Copyright
Copyright © Cambridge University Press 1986

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