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Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

  • D. C. De Graaf (a1), P. Berghen (a1), L. Moens (a2), T. M. De Marez (a1), S. Raes (a1), M. L. Blaxter (a3) and J. Vercruysse (a1)...

Summary

Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.

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Corresponding author

*Corresponding author: University of Gent, Faculty of Veterinary Medicine, Department of Parasitology, Salisburylaan 133, B-9820 Merelbeke, Belgium. Tel: + 32 9 264 7400. Fax: +32 9 264 74 96.

References

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Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

  • D. C. De Graaf (a1), P. Berghen (a1), L. Moens (a2), T. M. De Marez (a1), S. Raes (a1), M. L. Blaxter (a3) and J. Vercruysse (a1)...

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