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Ion channels of Fasciola hepatica incorporated into planar lipid bilayers

  • J. H. JANG (a1), S. D. KIM (a1), J. B. PARK (a2), S. J. HONG (a3) and P. D. RYU (a1)...

Abstract

Ion channels are important target sites of anthelmintics, but little is known about those in Fasciola hepatica. In this work, we applied a planar lipid bilayer technique to characterize the properties of single ion channels in F. hepatica. Under a 200/40 mM KCl gradient, a large conductance channel of 251 pS was observed in 18% of the membranes studied. The channel was selective to K+ over Cl with a permeability ratio of K+ to Cl (PK/PCl) of 4·9. Open state probability (Po) of the channel was less than 0·5 and dependent on voltage (−60~+40 mV) and Ca2+ (~100 μM). The other two types of single channels observed in 11 and 5% of membranes, respectively, were a K+-permeable channel of 80 pS (PK/PCl=4·6) and a Cl-permeable channel of 64 pS (PK/PCl=0·058). Open state probability of both channels showed little voltage dependence. The results indicate that distinct single channels of 60~251 pS are present in relative abundance and, in addition, that the planar lipid bilayer technique can be a useful tool for the study of single ion channels in F. hepatica.

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Corresponding author

Laboratory of Pharmacology, College of Veterinary Medicine, Seoul National University, San 56-1 Sillim-dong Kwanak-gu, Seoul 151-742, Republic of Korea. Tel: +82 2 880 1254. Fax: +82 2 879 0378. E-mail: pandryu@plaza.snu.ac.kr

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REFERENCES

BLAIR, K. L., DAY, T. A., LEWIS, M. C., BENNETT, J. L. & PAX, R. A. (1991). Studies on muscle cells isolated from Schistosoma mansoni: a Ca2+-dependent K+ channel. Parasitology 102, 251258.
CHEN, M. G. & MOTT, K. E. (1990). Progress in assessment of morbidity due to Fasciola hepatica infection: a review of recent literature. Tropical Disease Bulletin 87, R1R38.
DAY, T. A., BENNETT, J. L. & PAX, R. A. (1992). Schistosoma mansoni: Patch-clamp study of a nonselective cation channel in the outer tegumental membrane of females. Experimental Parasitology 74, 348356.
DAY, T. A., HAITHCOCK, J., KIMBER, M. & MAULE, A. G. (2000). Functional ryanodine receptor channels in flatworm muscle fibres. Parasitology 120, 417422.
GROSMAN, C. & REISIN, I. L. (1995). Echinococcus granulosus: partial characterization of the conductive properties of two cation channels from protoscoleces of the ovine strain, reconstituted on planar lipid bilayers. Experimental Parasitology 81, 546555.
GROSMAN, C. & REISIN, I. L. (1997). Interconverting gating modes of a nonselective cation channel from the tapeworm Echinococcus granulosus reconstituted on planar lipid bilayers. Journal of Membrane Biology 158, 8794.
GUO, X., UEHARA, A., RAVINDRAN, A., BRYANT, S. H., HALL, S. & MOCZYDLOWSKI, E. (1987). Kinetic basis for insensitivity to tetrodotoxin and saxitoxin in sodium channels of canine heart and denervated rat skeletal muscle. Biochemistry 26, 75467556.
KIM, E., DAY, T. A., BENNET, J. L. & PAX, R. A. (1995). Cloning and functional expression of a Shaker-related voltage-gated potassium channel gene from Schistosoma mansoni (Trematoda: Digenea). Parasitology 110, 171180.
KIM, H. S., KAM, K. Y., RYU, P. D., HONG, S. J., JEON, J. S., JEON, B. H., KIM, K. J. & PARK, J. B. (2002). A gadolinium and PH-sensitive hyperpolarization-activated cation current in acutely isolated single neurons from Fasciola hepatica. Parasitology 125, 423430.
KOHN, A. B., LEA, J., ROBERTS-MISTERLY, J. M., ANDERSON, P. A. & GREENBERG, R. M. (2001 a). Structure of three high voltage-activated calcium channel alpha1 subunits from Schistosoma mansoni. Parasitology 123, 489497.
KOHN, A. B., ANDERSON, P. A., ROBERTS-MISTERLY, J. M. & GREENBERG, R. M. (2001 b). Schistosome calcium channel beta subunits. Unusual modulatory effects and potential role in the action of antischistosomal drug praziquantel. Journal of Biological Chemistry 276, 3687336876.
LABARCA, P. & LATORRE, R. (1992). Insertion of ion channels into planar lipid bilayers by vesicle fusion. Methods in Enzymology 207, 447463.
LATORRE, R., OBERHAUSER, A., LABARCA, P. & ALBAREZ, O. (1989). Varieties of calcium-activated potassium channels. Anuual Review of Physiology 51, 385399.
LOYACANO, A. F., WILLIAMS, J. C., GURIE, J. & DEROSA, A. A. (2002). Effect of gastrointestinal nematode and liver fluke infections on weight gain and reproductive performance of beef heifers. Veterinary Parasitology 107, 227234.
MARTIN, R. J. (1997). Modes of action of anthelmintic drugs. Veterinary Journal 154, 1134.
MARTIN, R. J., ROBERTSON, A. P. & BJON, H. (1997). Target sites of anthelmintics. Parasitology 114, S111S124.
MAS-COMA, M. S., ESTEBAN, J. G. & BARQUES, M. D. (1999). Epidemiology of human fascioliasis: a review and proposed new classification. Bulletin of the World Health Organization 77, 340346.
PAX, R. A., DAY, T. A., MILLER, C. L. & BENNETT, J. L. (1996). Neuromuscular physiology and pharmacology of parasitic flatworms. Parasitology 113 (Suppl.), S83S96.
ROBERTSON, A. P., MARTIN, R. J. & KUSEL, J. R. (1997). A vesicle preparation for resolving single-channel currents in tegument of male Schistosoma mansoni. Parasitology 115, 183192.
SILVA, C. L., CUNHA, V. M., MENDONCA-SILVA, D. L. & NOEL, F. (1998). Evidence for ryanodine receptors in Schistosoma mansoni. Biochemical Pharmacology 56, 9971003.
SUKHDEO, S. C., SUKHDEO, M. V. K. & METTRICK, D. F. (1988). Neurocytology of the cerebral ganglion of Fasciola hepatica (Platyhelminthes). Journal of Comparative Neurology 278, 337343.

Keywords

Ion channels of Fasciola hepatica incorporated into planar lipid bilayers

  • J. H. JANG (a1), S. D. KIM (a1), J. B. PARK (a2), S. J. HONG (a3) and P. D. RYU (a1)...

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