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Intestinal parasites in rural communities in Nan Province, Thailand: changes in bacterial gut microbiota associated with minute intestinal fluke infection

Published online by Cambridge University Press:  04 May 2020

Ajala Prommi ,
Pinidphon Prombutara ,
Dorn Watthanakulpanich ,
Poom Adisakwattana ,
Teera Kusolsuk ,
Tippayarat Yoonuan ,
Akkarin Poodeepiyasawat ,
Nirundorn Homsuwan ,
Samreong Prummongkol  and
Malee Tanita
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Ajala Prommi
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Pinidphon Prombutara
Affiliation:
Faculty of Science, Omics Science & Bioinformatics Center, Chulalongkorn University, Bangkok, Thailand Faculty of Science, Microbiome Research Unit for Probiotics in Food and Cosmetics, Chulalongkorn University, Bangkok, Thailand
Dorn Watthanakulpanich
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Poom Adisakwattana
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Teera Kusolsuk
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Tippayarat Yoonuan
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Akkarin Poodeepiyasawat
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Nirundorn Homsuwan
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Samreong Prummongkol
Affiliation:
Faculty of Tropical Medicine, Mahidol Bangkok School of Tropical Medicine, Mahidol University, Bangkok, Thailand
Malee Tanita
Affiliation:
Saen Thong Health Promoting Hospital, Tha Wang Pha, Nan, Thailand
Sungkhom Rattanapikul
Affiliation:
Saen Thong Health Promoting Hospital, Tha Wang Pha, Nan, Thailand
Chuanphot Thinphovong
Affiliation:
Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand
Anamika Kritiyakan
Affiliation:
Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand
Serge Morand
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand Faculty of Veterinary Technology, CNRS ISEM – CIRAD ASTRE, Kasetsart University, Bangkok, Thailand
Kittipong Chaisiri*
Affiliation:
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
*
Author for correspondence: Kittipong Chaisiri, E-mail: kittipong.cha@mahidol.ac.th
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Abstract

Gastrointestinal helminth infection likely affects the gut microbiome, in turn affecting host health. To investigate the effect of intestinal parasite status on the gut microbiome, parasitic infection surveys were conducted in communities in Nan Province, Thailand. In total, 1047 participants submitted stool samples for intestinal parasite examination, and 391 parasite-positive cases were identified, equating to an infection prevalence of 37.3%. Intestinal protozoan species were less prevalent (4.6%) than helminth species. The most prevalent parasite was the minute intestinal fluke Haplorchis taichui (35.9%). Amplicon sequencing of 16S rRNA was conducted to investigate the gut microbiome profiles of H. taichui-infected participants compared with those of parasite-free participants. Prevotella copri was the dominant bacterial operational taxonomic unit (OTU) in the study population. The relative abundance of three bacterial taxa, Ruminococcus, Roseburia faecis and Veillonella parvula, was significantly increased in the H. taichui-infected group. Parasite-negative group had higher bacterial diversity (α diversity) than the H. taichui-positive group. In addition, a significant difference in bacterial community composition (β diversity) was found between the two groups. The results suggest that H. taichui infection impacts the gut microbiome profile by reducing bacterial diversity and altering bacterial community structure in the gastrointestinal tract.

Keywords

Gut microbiomeHaplorchis taichuihelminthminute intestinal flukeThailand
Type
Research Article
Information
Parasitology , Volume 147 , Issue 9 , August 2020 , pp. 972 - 984
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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