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The intestinal milieu influences the immunoproteome of male and female Heligmosomoides polygyrus bakeri L4 stage

Published online by Cambridge University Press:  30 July 2020

Marta Maruszewska-Cheruiyot Open the ORCID record for Marta Maruszewska-Cheruiyot [Opens in a new window] ,
Katarzyna Donskow-Łysoniewska ,
Katarzyna Krawczak ,
Ludmiła Szewczak ,
Ewa Joachimiak Open the ORCID record for Ewa Joachimiak [Opens in a new window]  and
Maria Doligalska
Marta Maruszewska-Cheruiyot*
Affiliation:
Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland
Katarzyna Donskow-Łysoniewska
Affiliation:
Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland
Katarzyna Krawczak
Affiliation:
Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland
Ludmiła Szewczak
Affiliation:
Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland
Ewa Joachimiak
Affiliation:
Laboratory of Cytoskeleton and Cilia Biology, Department of Cell Biology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland
Maria Doligalska
Affiliation:
Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland
*
Author for correspondence: Marta Maruszewska-Cheruiyot, E-mail: mmaruszewska@biol.uw.edu.pl
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Abstract

The gastrointestinal nematode Heligmosomoides polygyrus bakeri shows enhanced survival in mice with colitis. As the antibody response plays an important role in antiparasitic immunity, antibodies against male and female L4 H. polygyrus were examined in mice with and without colitis. Levels of specific antibodies in the mucosa and serum were determined by enzyme-linked immunosorbent assay and immunogenic proteins of male and female parasites were identified using 2D electrophoresis and mass spectrometry. The function of identified proteins was explored with Blast2Go. Nematodes in mice with colitis induced higher levels of specific immunoglobulin G (IgG1) and IgA, a lower level of IgE in the small intestine and a higher level of IgE in serum against female L4. Infected mice with colitis recognized 12 proteins in male L4 and 10 in female L4. Most of the recognized proteins from male L4 were intermediate filament proteins, whereas the proteins from female L4 were primarily actins and galectins. Nematodes from mice with colitis were immunogenically different from nematodes from control mice. This phenomenon gives new insights into helminth therapy as well as host–parasite interactions.

Keywords

ColitisHeligmosomoides polygyrus bakeriimmunogenic proteinsmass spectrometrytwo-dimensional electrophoresis
Type
Research Article
Information
Parasitology , Volume 147 , Issue 13 , November 2020 , pp. 1480 - 1487
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

Present address: Laboratory of Parasitology, General Karol Kaczkowski Military Institute of Hygiene and Epidemiology, Warsaw, Poland.

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