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The initial stages of infection of cattle cells with Theileria parva sporozoites in vitro

Published online by Cambridge University Press:  06 April 2009

D. A. Stagg ,
T. T. Dolan ,
B. L. Leitch  and
A. S. Young
D. A. Stagg
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
T. T. Dolan
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
B. L. Leitch
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
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Summary

Large numbers of Theileria parva sporozoites were separated from Rhipicephalus appendiculatus adult ticks by filtration and were then concentrated by centrifugation. The sporozoites were incubated at 37 °C with leucocytes from 6 cattle of Bos indicus and B. taurus types. Giemsastained smears and living preparations under interference contrast microscopy were used to follow the course of the infection of the leucocytes with sporozoites. Sporozoites were seen to attach rapidly to about 25% the leucocytes which they penetrated. After penetration by the sporozoites the morphology of the cells changed to show an increase in cytoplasm and an enlargement of the Golgi apparatus, with which the parasite appeared to become associated. The early intracellular or preschizont stages resembled Babesia parasites. From day 3, the parasite showed the typical morphology of the macroschizont of T. parva. Multiple infections were frequent and up to 8 schizonts were observed arranged around the Golgi apparatus. Multiply infected cells did not survive in culture but some of the cells infected with a single parasite divided to produce 2 infected daughter cells and infected lymphoblastoid cell lines were established in all 21 attempts.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 1 , August 1981 , pp. 191 - 197
Copyright
Copyright © Cambridge University Press 1981

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References

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