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Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype

Published online by Cambridge University Press:  06 April 2009

P. A. Conrad ,
C. L. Baldwin ,
W. C. Brown ,
B. Sohanpal ,
T. T. Dolan ,
B. M. Goddeeris ,
J. C. Demartini  and
O. K. Ole-Moiyoi
P. A. Conrad
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
C. L. Baldwin
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
W. C. Brown
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
B. Sohanpal
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
T. T. Dolan
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
B. M. Goddeeris
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
J. C. Demartini
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
O. K. Ole-Moiyoi
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
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Summary

Different stocks and stabilates within a stock of Theileriaparva were analysed for genotypic differences and for their effect on the expression of host cell surface antigens following infection of BoT8+ T lymphocyte clones. The parasites were characterized in vitro by hybridization of T. parva- specific DNA probes to Southern blots of endonuclease-digested DNA from the infected T cell clones. Phenotypic changes in the host lymphoblastoid cells before and after infection were examined using lineage-specific monoclonal antibodies which reacted with the differentiation antigens BoT2, BoT4, Bo6, BoT8 and a null cell marker on bovine T cells. Expression of Class I and Class II major histocompatibility complex (MHC) antigens on the cell populations was also assessed. Results of this study indicate that genotypically different parasites exist among and within T. parva stabilates and that the expression of Bo6, BoT8 and the null cell marker was differentially altered by infection with parasites from different stocks or from different stabilates of the same stock. Expression of Class II antigens was significantly increased after infection. Moreover, clones that were derived from the same cell line but had genotypically distinct T. parva parasites, also showed differences in expression of Bo6 and BoT8 and the null cell marker.

Keywords

Theileria parvaT lymphocytesdifferentiation antigenmajor histocompatibility antigensDNA probes
Type
Research Article
Information
Parasitology , Volume 99 , Issue 2 , October 1989 , pp. 205 - 213
Copyright
Copyright © Cambridge University Press 1989

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