Skip to main content Accessibility help
×
Home

Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype

  • P. A. Conrad (a1), C. L. Baldwin (a1), W. C. Brown (a1), B. Sohanpal (a1), T. T. Dolan (a1), B. M. Goddeeris (a1), J. C. Demartini (a1) and O. K. Ole-Moiyoi (a1)...

Summary

Different stocks and stabilates within a stock of Theileriaparva were analysed for genotypic differences and for their effect on the expression of host cell surface antigens following infection of BoT8+ T lymphocyte clones. The parasites were characterized in vitro by hybridization of T. parva- specific DNA probes to Southern blots of endonuclease-digested DNA from the infected T cell clones. Phenotypic changes in the host lymphoblastoid cells before and after infection were examined using lineage-specific monoclonal antibodies which reacted with the differentiation antigens BoT2, BoT4, Bo6, BoT8 and a null cell marker on bovine T cells. Expression of Class I and Class II major histocompatibility complex (MHC) antigens on the cell populations was also assessed. Results of this study indicate that genotypically different parasites exist among and within T. parva stabilates and that the expression of Bo6, BoT8 and the null cell marker was differentially altered by infection with parasites from different stocks or from different stabilates of the same stock. Expression of Class II antigens was significantly increased after infection. Moreover, clones that were derived from the same cell line but had genotypically distinct T. parva parasites, also showed differences in expression of Bo6 and BoT8 and the null cell marker.

Copyright

References

Hide All
Allsopp, B. A. & Allsopp, M. T. E. P. (1988). Theileria parva: genomic DNA studies reveal intra-specific sequence diversity. Molecular and Biochemical Parasitology 28, 7784.
Baldwin, C L., Black, S. J., Brown, W. C., Conrad, P. A., Goddeeris, B. M., Kinuthia, S. P., Lalor, P. A., Machugh, N. D., Morrison, W. I., Morzaria, S. P, Naessens, J. & Newson, j. (1988 a). Bovine T-cells, B- cells and null cells are transformed by the protozoan parasite Theileria parva. Infection and Immunity 56, 462–7.
Baldwin, C. L., Goddeeris, B. M. & Morrison, W. I. (1987). Bovine helper T-cell clones specific for lymphocytes infected with Theileria parva (Muguga). Parasite Immunology 9, 499513.
Baldwin, C. L., Machugh, N. D., Ellis, J. A., Naessens, J., Newson, J. & Morrison, W. I. (1988 b). Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: tissue distribution and functional characteristics of the target antigens. Immunology 63, 439–46.
Baldwin, C. L., Morrison, W. I. & Naessens, J. (1988 c). Differentiation antigens and functional characteristics of bovine leukocytes. In Differentiation Antigens in Lymphohemopoietic Tissues (ed. Miyasaka, M. & Trnka, Z)., pp. 455–98. New York and Basel: Marcel Dekker Inc.
Baldwin, C. L., Teale, A. J., Naessens, J., Goddeeris, B. M., Machugh, N. D. & Morrison, W. I. (1986). Characterization of a subset of bovine T lymphocytes expressing BoT4 by monoclonal antibodies and function: similarity to lymphocytes defined by human T4 and murine L3T4. Journal of Immunology 136, 4385–91.
Brown, C. G. D. (1983). Theileria. In In vitro Cultivation of Protozoan Parasites (ed. Jensen, J. B.), pp. 243–8. Boca Raton, Florida: CRC Press Inc.
Brown, C. G. D., Stagg, D. A., Purnell, R. E., Kanhai, G. K. & Payne, R. C. (1973). Infection and transformation of bovine lymphoid cells in vitro by infective particles of Theileria parva. Nature, London 245, 101–3.
Brown, W. C. & Grab, D. J. (1985). Biological and biochemical characterization of bovine interleukin 2. Studies with cloned bovine T cells. Journal of Immunology 133, 3184–9.
Cillo, C., Mach, J. P., Schreyer, M. & Carrel, S. (1984). Antigenic heterogeneity of clones and subclones from human melanoma cell lines demonstrated by a panel of monoclonal antibodies and flow microfluorometry analysis. International Journal of Cancer 34, 1120.
Conrad, P. A., Iams, K., Brown, W. C., Sohanpal, B. & Ole-Moiyoi, O. K. (1987). DNA probes detect genomic diversity in Theileria parva stocks. Molecular and Biochemical Parasitology 25, 213–26.
Conrad, P. A., Ole-Moiyoi, O. K., Baldwin, C. L., Dolan, T. T., O'Callaghan, C. J., Njamunggeh, R. E. G., Grootenhuis, J. G., Stagg, D. A., Leitch, B. L. & Young, A. S. (1989). Characterization of buffalo- derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology 98, 179–88.
Conrad, P. A., Stagg, D. A., Grootenhijis, J. G., Irvin, A. D., Newson, J., Njamunggeh, R. E. G., Rossiter, P. B. & Young, A. S. (1987). Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies. Parasitology 94, 413–23.
Dobbelaere, D. A. E., Spooner, P. R., Barry, W. C. & Irvin, A. D. (1984). Monoclonal antibody neutralizes the sporozoite stage of different Theileria parva stocks. Parasite Immunology 6, 361–70.
Duport Guerry, D. IV, Zehnebot, L. M., Mitchel, K. F., Zmijewski, C. M. & Lask, E. J. (1984). HLA-DR histocompatibility leukocyte antigens permit cultured human melanoma cells from early but not advanced disease to stimulate autologous lymphocytes. Journal of Clinical Investigation 73, 267–71.
Ellis, J. A., Baldwin, C. L., Machugh, N. D., Bensaid, A., Teale, A. J., Goddeeris, B. M. & Morrison, W. I. (1986). Characterization by monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology 58, 351–8.
Enery, D. L., Eugui, E. M., Nelson, R. T. & Tenywa, T. (1981). Cell mediated immune responses to Theileria parva (East Coast fever) during immunization and lethal infections in cattle. Immunology 43, 323–36.
Emery, D. L., Machugh, N. D. & Morrison, W. I. (1989). Theileria parva infects bovine T-lymphocytes in vivo and in vitro and induces co-expression of BoT4 and BoT8. Parasite Immunology (in the Press).
Eugui, E. M. & Emery, D. L. (1981). Genetically restricted cell mediated cytotoxicity in cattle immune to Theileria parva. Nature, London 290, 251–4.
Goddeeris, B. M., Morrison, W. I., Teale, A. J., Bensaid, A. & Baldwin, C. L. (1986). Bovine cytotoxic T cell specific for cells infected with the protozoan parasite Theileria parva: parasite strain specificity and class 1 major histocompatibility complex restriction. Proceedings of the National Academy of Sciences, USA 83, 5238–42.
Hulliger, L., Wilde, J. K. H., Brown, C. G. D. & Turner, L. (1964). Mode of multiplication of Theileria. Parasitology 80, 571–9.
Koch, H. T., Ocama, J. G. R., Munatswa, F. C., Byrom, B., Norval, R. A. I., Spooner, P. R., Conrad, P. A. & Irvin, A. D. (1987). Isolation and characterization of bovine Theileria parasites in Zimbabwe. Veterinary Parasitology 28, 1931.
Kurtti, T. J., Munderloh, U. G., Irvin, A. D. & Büscher, G. (1981). Theileria parva: early events in the development of bovine lymphoblastoid cell lines persistently infected with macroshizonts. Experimental Parasitology 52, 280–90.
Lalor, P. A., Morrison, W. I., Goddeeris, B. M., Jack, R. J. & Black, S. J. (1986). Monoclonal antibodies identify phenotypically and functionally distinct cell types in the bovine lymphoid system. Veterinary Immunology and Immunopathology 13, 121–40.
Malmquist, W. A., Nyindo, M. B. A. & Brown, C. G. D. (1970). East Coast fever: cultivation in vitro of bovine spleen cell lines infected and transformed by Theileria parva. Tropical Animal Health and Production 2, 139–45.
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning, A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
Morrison, W. I., Goddeeris, B. M. & Teale, A. J. (1987). Bovine cytotoxic T cell clones which recognize lymphoblasts infected with two antigenically different stocks of the protozoan parasite Theileria parva. European Journal of Immunology 17, 1703–9.
Morrison, W. I., Machugh, N. D., Bensaid, A., Goddeeris, B. M., Teale, A. J. & Mckeever, D. J. (1989). A monoclonal antibody which reacts specifically with a population of bovine lymphocytes lacking B cell and T cell markers. In Proceedings of 9th International Conference on Lymphatic Tissues and Germinal Centres in Immune Reactions, Oslo 1987 (in the Press).
Ruiter, D. J., Bergman, W., Welvaart, K., Scheffer, E., Van Vloten, W. A., Russo, C. & Ferrone, S. (1984). Immunohistochemical analysis of malignant melanomas and nevocellular nevi with monoclonal antibodies to distinct monomorphic determinants of HLA antigens. Cancer Research 44, 3390–5.
Shiels, B., Mcdougall, C., Tait, A. & Brown, C. G. D. (1986). Antigenic diversity of Theileria annulata macroshizonts. Veterinary Parasitology 21, 110.
Teale, A. J., Kemp, S. J., Young, F. & Spooner, R. L. (1983). Selection by major histocompatibility type (BoLA) of lymphoid cells derived from a bovine chimera and transformed by Theileria parasites. Parasite Immunology 5, 329–36.

Keywords

Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype

  • P. A. Conrad (a1), C. L. Baldwin (a1), W. C. Brown (a1), B. Sohanpal (a1), T. T. Dolan (a1), B. M. Goddeeris (a1), J. C. Demartini (a1) and O. K. Ole-Moiyoi (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed