Hostname: page-component-77c89778f8-vsgnj Total loading time: 0 Render date: 2024-07-20T07:43:05.233Z Has data issue: false hasContentIssue false
  • English
  • Français

Induction of protective immunity and modulation of granulomatous hypersensitivity in mice using PIII, an anionic fraction of Schistosoma mansoni adult worm

Published online by Cambridge University Press:  01 July 1997

C. HIRSCH ,
C. S. ZOUAIN ,
J. B. ALVES  and
A. M. GOES
C. HIRSCH
Affiliation:
Departamento de Bioquímica e Imunologia,
C. S. ZOUAIN
Affiliation:
Departamento de Bioquímica e Imunologia,
J. B. ALVES
Affiliation:
Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
A. M. GOES
Affiliation:
Departamento de Bioquímica e Imunologia,
Get access Rights & Permissions [Opens in a new window]

Abstract

This study was performed in order to define Schistosoma mansoni antigens that are able to function as modulator agents in the granulomatous hypersensitivity to parasite eggs in BALB/c and C57BL/6 mice. A fraction of S. mansoni, designated PIII, derived from adult worm antigen preparation (SWAP) was obtained using anion-exchange chromatography on an FPLC system. Immunization of mice with PIII in the presence of Corynebacterium parvum and Al(OH)3 as adjuvant induced an immune response in these animals as determined by ELISA and spleen cell proliferation assays against S. mansoni antigens SEA, SWAP and PIII. In addition, PIII caused a significant degree of protection against a challenge infection in immunized mice as observed by the decrease on worm burden recovered from the portal system. We also showed that PIII profoundly inhibited the vigorous anamnestic granulomatous response to eggs in the liver and lungs. This suppression correlated with a significant decrease in granuloma size. From these results we conclude that the PIII preparation contains antigens that can mediate protective anti-parasite immunity and downregulate granulomatous hypersensitivity to S. mansoni eggs.

Keywords

Schistosoma mansonimodulationgranulomawormimmunity
Type
Research Article
Information
Parasitology , Volume 115 , Issue 1 , July 1997 , pp. 21 - 28
Copyright
1997 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)