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In vitro suppression of segmentation in Echinococcus multilocularis with morphological transformation of protoscoleces into monozoic adults

Published online by Cambridge University Press:  06 April 2009

J. D. Smyth  and
Zena Davies
J. D. Smyth
Affiliation:
Department of Zoology & Applied Entomology, Imperial College, London, SW7 2BB
Zena Davies
Affiliation:
Department of Zoology & Applied Entomology, Imperial College, London, SW7 2BB
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Extract

When protoscoleces of Echinococcus multilocularis were cultured in vitro, under axenic conditions in either monophasic or diphasic media, segmentation was suppressed in most organisms, some 70–80% of which developed into unsegmented, monozoic forms with a complete set of sexually mature male and female genitalia. The most striking feature of monozoic worms was the large lateral swelling produced by the cirrus sac the effect being to produce organisms with an unusual asymmetric shape. Worms which did not become monozoic either (a) underwent some somatic growth, developed two sets of genitalia and became ‘pseudosogmented’, i.e. with the inter-proglottid membranes absent or poorly defined, or (b) became vesicular or abnormal. The mechanisms which could be involved in the suppression of somatic growth and the induction of the monozoic condition, are examined in terms of cell lineage. The possible significance of these results in understanding the evolution of the cestodes is discussed.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 1 , August 1975 , pp. 125 - 136
Copyright
Copyright © Cambridge University Press 1975

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References

REFERENCES

Bolla, R. I. & Roberts, L. S. (1971). Developmental physiology of cestodes. IX. Cytological characteristics of the germinative region of Hymenolepis diminuta. Journal of Parasitology 57, 267–77.CrossRefGoogle ScholarPubMed
Von Bonsdorff, C.-H., Forssten, T., Gustafsson, M. K. S. & Wikgren, B.-J. (1971) Cellular composition of plerocercoids of Diphyllobothrium dendriticum (Cestoda). Acta Zoologica Fennica 132, 1–25.Google Scholar
Burnett, A. L. & Diehl, N. A. (1964). The nervous system of Hydra. III. The initiation of sexuality with special reference to the nervous system. Journal of Experimental Zoology 157 237–50.Google Scholar
Cameron, I. L. & Jeter, J. R. (1971). Relationship between cell proliferation and cyto differentiation in embryonic chick tissues. In Developmental Aspects of the Cell Cycle (ed Cameron, I. L., Padilla, G. M. and Zimmerman, A. M.), pp. 191–222. Academic Press.Google Scholar
Corff, S. (1971). Mitosis and differentiation in Hydra morphogenesis. In Developmental Aspects of the Cell Cycle (ed. Cameron, I. L., Padilla, G. M. and Zimmerman, A. M.), pp 93–125. Academic Press.CrossRefGoogle Scholar
Douglas, L. P. (1961). The development of organ systems in nematotaeniid cestodes 1. Early histogenesis and formation of reproductive structures in Baerietta diana (Helfer, 1948). Journal of Parasitology 47, 669–80.Google Scholar
Freeman, R. S. (1973). Ontogeny of cestodes and its bearing on their phylogeny and systematics. Advances in Parasitology 11, 481–557.Google Scholar
Hay, E. D. (1958). The fine structure of blastema cells and differentiating cartilage cells inregenerating limbs of Amblystoma larvae. Journal of Biophysical and Biochemical Cytology 4, 538–92.Google Scholar
Llewellyn, J. (1965). The evolution of parasitic platyhelminths. Symposia of the Britis Society for Parasitology 3, 47–78.Google Scholar
Lubinsky, G. (1960). The maintenance of Echinococcus multilocularis sibricensis without the definitive host. Canadian Journal of Zoology 38, 1117–25.Google Scholar
Mackiewicz, J. S. (1972). Caryophyllidea (Cestoidea): a review. Experimental Parasitology 31, 417–512.Google Scholar
Malmberg, G. (1974). On the larval protonephridial system of Gyrocotyle and the evolution of cercomeromorphae (Platyhelminthes). Zoologica Scripta 3, 65–81.Google Scholar
Norman, L. & Kagan, I. G. (1961). The maintenance of Echinococcus multilocularis in gerbils (Meriones unguiculatus) by intraperitoneal inoculation. Journal of Parasitology 47, 870–4.Google Scholar
Smyth, J. D. (1964). The biology of the hydatid organisms. Advances in Parasitology 2, 169–219.Google Scholar
Smyth, J. D. (1967). Studies on tapeworm physiology. IX. In vitro cultivation of Echinococcus granulosus from the protoscolex to the strobilate stage. Parasitology 57, 111–33.Google Scholar
Smyth, J. D. (1971). Development of monozoic forms of Echinococcus granulosus during in vitro culture. International Journal for Parasitology 1, 121–4.Google Scholar
Smyth, J. D. (1972). Changes in the digestive-absorptive surface of cestodes during larval/adult differentiation. Symposia of the British Society for Parasitology 10, 41–70.Google Scholar
Smyth, J. D. & Davies, Z. (1974). In vitro culture of the strobilar stage of Echinococcus granulosus (sheep strain): a review of basic problems and results. International Journal for Parasitology 64, 631–44.Google Scholar
Sulgostowska, P. (1972). The development of organ systems in cestodes. I. A study of histology of Hymenolepis diminuta (Rudolphi, 1819) Hymenolepididae. Acta Parasitologica Polonica 20, 449–62.Google Scholar
Sulgostowska, T. (1974). The development of organ systems in cestodes. II. Histogenesis of the reproductive system in Hymenolepis diminuta (Rudolphi, 1819) (Hymenolepididae). Acta Parasitologica Polonica 22, 179–90.Google Scholar
Wikgren, B.-J. P. & Gustafsson, M. K. S. (1971). Cell proliferation and histogenesis in diphyllobothrid tapeworms (Cestoda). Acta Academiae Aboensis Ser. B. 31(2), 1–10.Google Scholar
Wikgren, B.-J. P. & Knuts, G. M. (1970). Growth of subtegumental tissue in cestodes by cell migration. Acta Academiae Aboensis Ser. B 30(16), 1–6.Google Scholar
Yamashita, J., Ohbayashi, M. & Kitamura, Y. (1958). Differences in development of the tapeworm stage between Echinococcus granulosus (Batsch, 1786) and E. multilocularis (Leuckart, 1863). Japanese Journal of Veterinary Research 6, 226–9.Google Scholar