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In vitro isolation of Plasmodium knowlesi merozoites using polycarbonate sieves

Published online by Cambridge University Press:  06 April 2009

E. D. Dennis ,
G. H. Mitchell ,
G. A. Butcher  and
S. Cohen
E. D. Dennis
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT
G. H. Mitchell
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT
G. A. Butcher
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT
S. Cohen
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT
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Extract

A culture chamber fitted with a polycarbonate sieve has been used to isolate Plasmodium knowlesi merozoites as they are released from schizonts. A 3 μm pore-size sieve allows passage of normal erythrocytes and red cells containing rings and trophozoites and can be used to concentrate schizonts from a mixed cell population. A 2 μm pore-size sieve retains normal and parasitized cells and provides uncontaminated merozoites in high yield (5 × 1010 merozoites per ml schizonts). Merozoite viability diminishes rapidly during 30 min after isolation. These preparations should prove valuable for studies of the biochemical, physiological and antigenic properties of this transient extracellular phase of the malaria parasite.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 3 , December 1975 , pp. 475 - 481
Copyright
Copyright © Cambridge University Press 1975

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References

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