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Immunochemical analysis of Taenia taeniaeformis antigens expressed in Escherichia coli

Published online by Cambridge University Press:  06 April 2009

D. D. L. Bowtell ,
R. B. Saint ,
M. D. Rickard  and
G. F. Mitchell
D. D. L. Bowtell
Affiliation:
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, AU
R. B. Saint
Affiliation:
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, AU
M. D. Rickard
Affiliation:
University of Melbourne, Veterinary Clinical Centre, Werribee, AU
G. F. Mitchell
Affiliation:
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, AU
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Summary

Previously we reported the isolation of several Escherichia coli clones expressing fragments of Taenia taeniaeformis antigens as β-galactosidase fused proteins (Bowtell, Saint, Rickard & Mitchell, 1984). Here we describe the isolation of additional antigen-expressing clones from a larval cDNA library and the assignment of these clones to 7 antigen families. These were isolated with a polyspecific rabbit antiserum raised to the oncosphere. Since this serum was capable of reacting with a large number of antigens, it was important to develop techniques for rapidly determining the identity of the native T. taeniaeformis molecule corresponding to a cloned antigen gene. These included active immunization of rabbits with fused proteins and several techniques involving affinity purification on immobilized fused proteins. The reactivity of the antigen-positive clones with sera from humans infected with related parasites was also assessed. Finally, immunization of mice with several fused proteins failed to protect against subsequent infection, although antigens previously identified as candidate host-protective antigens (Bowtell, Mitchell, Anders, Lightowlers & Rickard, 1983) have yet to be identified in the expression library.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 3 , December 1986 , pp. 599 - 610
Copyright
Copyright © Cambridge University Press 1986

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References

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