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Identification of non-cross-reacting antigens of Onchocerca volvulus with lymphatic filariasis serum pools

Published online by Cambridge University Press:  06 April 2009

E. Lobos  and
N. Weiss
E. Lobos
Affiliation:
Laboratory of Immunoparasitology, Swiss Tropical Institute, Socinstrasse 57, 4051 Basel, Switzerland
N. Weiss
Affiliation:
Laboratory of Immunoparasitology, Swiss Tropical Institute, Socinstrasse 57, 4051 Basel, Switzerland
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Summary

Onchocerca volvulus proteins labelled with 125I were immunoprecipitated with onchocerciasis and lymphatic filariasis human serum pools in order to differentiate between cross-reacting and non-cross-reacting antigens. Analysis of the immunoprecipitates by two-dimensional gel electrophoresis revealed that all high molecular weight (Mr) O. volvulus antigens cross-reacted with the lymphatic filariasis serum pools. We observed, however, that at least 8 0. volvulus antigens were specifically immunoprecipitated only by the onchocerciasis serum pools, with Mr ranging from 20000 up to 43000 Daltons. These results suggest that the lower MrO. volvulus antigens are more species specific than the other antigens. The significance of these findings for the immunodiagnosis of onchocerciasis is discussed.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 389 - 399
Copyright
Copyright © Cambridge University Press 1986

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References

Ambroise-Thomas, P. (1974). Immunological diagnosis of human filariasis: present possibilities, difficulties and limitations. Acta Tropica 38, 108–28.Google Scholar
Forchhammer, J. (1979). RNA Tumor Viruses. A Laboratory Manual in Advanced Techniques. Max-Planck-Institut für Molekulare Genetik.Google Scholar
Forsyth, K. P., Copemak, D. B., Anders, R. F. & Mitchell, G. F. (1981). The major radioiodinated cuticular antigens of Onchocerca gibsoni microfilariae are neither species nor onchocerca specific. Acta Tropica 38, 348–52.Google ScholarPubMed
Fujita, K., Tanaka, H., Sasa, M., Schichinone, K., Asai, Y. & Kurokawa, Y. (1970). Cross-reaction among filarial species in hemagglutination test. Japanese Journal of Experimental Medicine 40, 67–77.Google Scholar
Greenwood, F. C., Hunter, W. M. & Glover, J. S. (1963). The preparation of 131I-labelled human growth hormone of high specific radioactivity. Journal of Biochemistry 89, 114–19.CrossRefGoogle Scholar
Johnson, B. F. (1982). Enhanced resolution in two dimensional electrophoresis of low molecular weight proteins while using enlarged gels. Analytical Biochemistry 127, 235–46.CrossRefGoogle ScholarPubMed
Kagan, I. G. & Norman, L. (1974). Manual of Clinical Microbiology, (ed. Lenette, E. H., Spaulding, E. H. and Traunt, J. P.), pp. 645–63. Washington D.C.: American Society for Microbiology.Google Scholar
Kaushal, N. A., Hussain, R. & Ottessen, E. A. (1984). Excretory and somatic antigens in the diagnosis of human filariasis. Clinical and Experimental Immunology 56, 567–76.Google ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227, 680–5.Google Scholar
Lobos, E. & Weiss, N. (1985). Immunochemical comparison between worm extracts of Onchocerca volvulus from savanna and rain forest. Parasite Immunology 7, 333–47.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. R., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
McGreevy, P. B., Ratiwayanto, S., Sekar, T., McGreevy, M. M., Piessens, P. W., Koiman, I., Saroso, J. S. & Dennis, D. T. (1980). Immune responses in human infections with Brugia malayi: relationship between anti-sheath antibodies and amicrofilaremia in natives living in an endemic area of South Kalimantan, Borneo. American Journal of Tropical Medicine and Hygiene 29, 553–62.Google Scholar
Nelson, G. S. (1981). Issues in filariosis – a century of enquiry and century of failure. Acta Tropica 38, 197–204.Google Scholar
O'Farrell, P. (1975). High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4407–21.CrossRefGoogle ScholarPubMed
Ottesen, E. A., Weller, P. F. & Heck, L. (1977). Specific cellular immune unresponsiveness in human filariasis. Immunology 33, 413–21.Google Scholar
Ottesen, E. A., Weller, P. F., Lunde, M. N. & Hussain, R. (1982). Endemic filariasis on a Pacific Island. II. Immunologic aspects: immunoglobulin, complement, and specific antifilarial IgG, IgM, and IgE antibodies. American Journal of Tropical Medicine and Hygiene 31, 953–61.Google Scholar
Ouassi, A., Des Moutis, J., Cornette, J., Pierce, R. & Capron, A. (1983). Detection of IgE-antibodies in onchocerciasis using semi-purified fraction from Dipetalonema vitae total antigen. International Archives of Allergy and applied Immunology 70, 231–7.CrossRefGoogle Scholar
Phillip, M., Gomez-Priego, A., Parkhouse, R. M. E., Davies, M. W., Clark, N. W. T., Ogilvie, B. M. & Beltrán-Hernández, F. (1984). Identification of an antigen of Onchocerca volvulus of possible diagnostic use. Parasitology 89, 295–309.Google Scholar
Piessens, W. F., McGreevy, P. B., Ratiwayanto, S., McGreevy, M., Piessens, P. W., Koiman, I., Saroso, J. S. & Dennis, D. T. (1980). Immune responses in human infections with Brugia malayi: Correlation of cellular and humoral reactions to microfilarial antigens with clinical status. American Journal of Tropical Medicine and Hygiene 29, 553–70.Google Scholar
Schulz-Key, H., Albiez, E. J. & Buettner, D. W. (1977). Isolation of living adult Onchocerca volvulus from nodules. Tropenmedizin und Parasitologie 28, 428–30.Google ScholarPubMed
Speiser, F. (1980). Application of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of filariasis and echinococcosis. Tropenmedizin und Parasitologie 31, 459–66.Google Scholar
Tanaka, H., Fujita, K., Sasa, M., Tagawa, M., Naito, M. & Kurokawa, K. (1970). Cross-reaction in complement fixation test among filaria species. Japanese Journal of Experimental Medicine 40, 47–58.Google ScholarPubMed
Voller, A. & De Savigny, D. (1981). Diagnostic serology of tropical parasitic diseases. Journal of Immunological Methods 46, 1–29.CrossRefGoogle ScholarPubMed
Weiss, N., Gualzata, M., Wyss, T. & Betschart, B. (1982). Detection of IgE binding Onchocerca volvulus antigens after electrophoretic transfer and immuno-enzyme reaction. Acta Tropica 39, 373–7.Google Scholar
World Health Organization (1976). Epidemiology of onchocerciasis. WHO Technical Report Series No. 597.Google Scholar
World Health Organization (1984). Application of monoclonal antibodies to filarial antigens. Bulletin 62 (5), 695–6.Google Scholar