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Identification of a novel PYP-1 gene in Sarcoptes scabiei and its potential as a serodiagnostic candidate by indirect-ELISA

Published online by Cambridge University Press:  08 November 2017

Jing Xu ,
Xing Huang ,
Manli He ,
Yongjun Ren ,
Nengxing Shen ,
Chunyan Li ,
Ran He ,
Yue Xie ,
Xiaobin Gu  and
Bo Jing
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Jing Xu
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Xing Huang
Affiliation:
Chengdu Agricultural College, Chengdu 611130, China
Manli He
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yongjun Ren
Affiliation:
Sichuan Animal Sciences Academy, Sichuan Chengdu 610066, China Animal Breeding and Genetics key Laboratory of Sichuan Province, Sichuan Chengdu 610066, China
Nengxing Shen
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Chunyan Li
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Ran He
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yue Xie
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Xiaobin Gu
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Bo Jing
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Xuerong Peng
Affiliation:
College of Science, Sichuan Agricultural University, Ya'an 625014, China
Guangyou Yang*
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
*
Author for Correspondence: Guangyou Yang, E-mail: guanyouyang@hotmail.com; guangyou1963@aliyun.com
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Abstract

Scabies is a parasitic disease caused by the ectoparasite Sarcoptes scabiei, affecting different mammalian species, including rabbits, worldwide. In the present study, we cloned and expressed a novel inorganic pyrophosphatase, Ssc-PYP-1, from S. scabiei var. cuniculi. Immunofluorescence staining showed that native Ssc-PYP-1 was localized in the tegument around the mouthparts and the entire legs, as well as in the cuticle of the mites. Interestingly, obvious staining was also observed on the fecal pellets of mites and in the integument of the mites. Based on its good immunoreactivity, an indirect enzyme-linked immunosorbent assay (ELISA) using recombinant Ssc-PYP-1 (rSsc-PYP-1) as the capture antigen was developed to diagnose sarcoptic mange in naturally infected rabbits; the assay had a sensitivity of 92·0% and specificity of 93·6%. Finally, using the rSsc-PYP-1-ELISA, the Ssc-PYP-1 antibody from 10 experimentally infected rabbits could be detected from 1 week post-infection. This is the first report of S. scabiei inorganic pyrophosphatase and the protein could serve as a potential serodiagnostic candidate for sarcoptic mange in rabbits.

Keywords

Sarcoptes scabieiinorganic pyrophosphatasefluorescence immunohistochemistryserodiagnosisindirect ELISA
Type
Research Article
Information
Parasitology , Volume 145 , Issue 6 , May 2018 , pp. 752 - 761
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Copyright
Copyright © Cambridge University Press 2017 

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