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Growth in vitro of Parvatrema timondavidi Bartoli 1963 (Trematoda: Gymnophallidae) from the metacercarial stage to egg production

Published online by Cambridge University Press:  06 April 2009

Kazuo Yasuraoka ,
Masayoshi Kaiho ,
Hidekazu Hata  and
Takuro Endo
Kazuo Yasuraoka
Affiliation:
Department of Parasitology, National Institute of Health, Tokyo, 141, Japan
Masayoshi Kaiho
Affiliation:
Department of Parasitology, National Institute of Health, Tokyo, 141, Japan
Hidekazu Hata
Affiliation:
Department of Parasitology, National Institute of Health, Tokyo, 141, Japan
Takuro Endo
Affiliation:
Department of Parasitology, National Institute of Health, Tokyo, 141, Japan
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Extract

Metacercariae of Parvatrema timondavidi were successfully cultivated in vitro to adults which produced eggs capable of embryonation and miracidial formation. The worms were grown in NCTC 109 supplemented with 20% inactivated bovine or chicken serum with a gas phase of 8% CO2 in air at 37 or 41°C. The use of the roller tube technique produced considerable promotion of growth in the worms. Heat inactivated bovine serum had a more salutary effect than unheated serum.

Type
Research Article
Information
Parasitology , Volume 68 , Issue 3 , June 1974 , pp. 293 - 302
Copyright
Copyright © Cambridge University Press 1974

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