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Genotyping of Theileria parva populations in vaccinated and non-vaccinated cattle in Malawi

Published online by Cambridge University Press:  11 April 2022

Elisha Chatanga ,
Yuma Ohari ,
Walter Muleya Open the ORCID record for Walter Muleya [Opens in a new window] ,
Kyoko Hayashida ,
Chihiro Sugimoto ,
Ken Katakura Open the ORCID record for Ken Katakura [Opens in a new window] ,
Nariaki Nonaka  and
Ryo Nakao Open the ORCID record for Ryo Nakao [Opens in a new window]
Elisha Chatanga
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Sapporo, Hokkaido 060-0818, Japan Department of Veterinary Pathobiology, Lilongwe University of Agriculture and Natural Resources, P.O. Box 219, Lilongwe, Malawi
Yuma Ohari
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Sapporo, Hokkaido 060-0818, Japan
Walter Muleya
Affiliation:
Department of Biomedical Sciences, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia
Kyoko Hayashida
Affiliation:
Division of Collaboration and Education, International Institute for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Sapporo, Hokkaido 001-0020, Japan
Chihiro Sugimoto
Affiliation:
Division of Collaboration and Education, International Institute for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Sapporo, Hokkaido 001-0020, Japan
Ken Katakura
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Sapporo, Hokkaido 060-0818, Japan
Nariaki Nonaka
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Sapporo, Hokkaido 060-0818, Japan
Ryo Nakao*
Affiliation:
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Sapporo, Hokkaido 060-0818, Japan
*
Author for correspondence: Ryo Nakao, E-mail: ryo.nakao@vetmed.hokudai.ac.jp
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Abstract

Theileria parva is an apicomplexan protozoan parasite that causes bovine theileriosis (East Coast Fever; ECF) in central, eastern and southern Africa. In Malawi, ECF is endemic in the northern and central regions where it has negatively affected the development of dairy industry. Despite its endemic status the genetic population structure of T. parva in Malawi is currently unknown. To obtain an understanding of T. parva in Malawi, we performed population genetics analysis of T. parva populations in cattle vaccinated with the Muguga cocktail live vaccine and non-vaccinated cattle using mini- and microsatellite markers covering all the four T. parva chromosomes. The T. parva Muguga strain was included in this study as a reference strain. Linkage disequilibrium was observed when all samples were treated as a single population. There was sub-structuring among the samples as shown by the principal coordinate analysis. Majority of the samples clustered with the T. parva Muguga reference strain suggesting that the isolates in Malawi are closely related to the vaccine component, which support the current use of Muguga cocktail vaccine to control ECF. The clustering of samples from non-endemic southern region with those from endemic central region suggests expansion of the distribution of T. parva in Malawi.

Keywords

GenotypingMalawipopulation structureTheileria parva
Type
Research Article
Information
Parasitology , Volume 149 , Issue 7 , June 2022 , pp. 983 - 990
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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