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The FMRFamide-like neuropeptide AF2 is present in the parasitic nematode Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

C. D. Keating ,
L. Holden-Dye ,
M. C. Thorndyke ,
R. G. Williams ,
A. Mallett  and
R. J. Walker
C. D. Keating
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
L. Holden-Dye
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
M. C. Thorndyke
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX
R. G. Williams
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
A. Mallett
Affiliation:
St Johns Institute of Dermatology, UMDS St Thomas' Hospital, London SE1 7EH
R. J. Walker
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX
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Summary

Peptides belonging to the FMRFamide family are widely distributed amongst invertebrates. We report here on the isolation of the FMRFamide neuropeptide AF2 (Lys-His-Glu-Tyr-Leu-Arg-Phe-NH2) from the parasitic nematode Haemonchus contortus. Immunocytochemical techniques showed that FMRFamide-like material was distributed in several regions of these organisms including nerve cords and cell bodies of the central nervous system. AF2 was isolated using a method that employed 6 steps of reverse-phase HPLC. The concentration of AF2 in this organism was approximately 30 pmol/g of nematode.

Keywords

Haemonchus contortusAF2FMRFamide-related peptidesimmunocytochemistryneuropeptides
Type
Research Article
Information
Parasitology , Volume 111 , Issue 4 , November 1995 , pp. 515 - 521
Copyright
Copyright © Cambridge University Press 1995

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