Skip to main content Accessibility help
×
Home

Epitopes expressed on very low Mr Schistosoma mansoni adult tegumental antigens conform to a general pattern of life-cycle cross-reactivity

  • A. J. G. Simpson (a1), P. Hagan (a1), F. Hackett (a1), P. Omer Ali (a1) and S. R. Smithers (a1)...

Summary

The relationship between antigens associated with the surface of newly transformed schistosomula of Schistosoma mansoni and the tegumental surface membrane of adult S. mansoni worms has been further explored. Immunoprecipitation of detergent-solubilized 125I-tegumental surface membrane antigens of adult S. mansoni with antibodies from mice vaccinated with highly irradiated S. mansoni cercariae revealed major antigens of Mr 32, 20, 15 and 8K. The Mr 32 and 20K antigens have been previously demonstrated to be antigenically and electrophoretically identical to major antigens on the schistosomulum surface. The Mr 15 and 8K antigens, on the other hand, have not been identified by the immunoprecipitation of 125I-schistosomulum surface antigens, although a distinct schistosomulum surface antigen of Mr 15K is precipitated by antibodies from mice vaccinated with highly irradiated cercariae. Nevertheless, it was shown that antibodies to the Mr 15 and 8K antigens were specifically absorbed from vaccinated mouse serum by intact, live schistosomula, demonstrating that the Mr 15 and 8K antigens are exposed on or released from the schistosomulum surface. In contrast, absorption of the antiserum with eggs failed to remove antibody against any of the four tegumental membrane antigens examined. The Mr 15 and 8K antigens were shown to be recognized via polypeptide epitopes and not periodate-sensitive carbohydrate epitopes, further emphasizing the similarity of these to the well-characterized Mr 32 and 20K tegumental surface membrane antigens. A general relationship between schistosomulum surface, adult tegumental membrane and egg antigens was demonstrated by ELISA, using antibodies raised against the three antigenic fractions. It was shown that both the egg and adult tegumental membrane antigens cross-react with the schistosomulum surface, but that the egg and adult membrane antigens exhibit very little, if any, mutual cross-reactivity. This antigen divergence possibly enables the host to dissociate pathological, anti-egg responses from potentially protective anti-membrane responses during the course of natural infection. It also suggests that adult membrane antigens could be used in an anti-schistosome vaccine without the possible complication of inducing pathological responses.

Copyright

References

Hide All
Fraker, P. J. & Speck, J. C. Jr. (1987). Protein and cell membrane iodination with a sparingly soluble chloramide, l,3,4,6-tetrachloro-3a,6a-diphenyl-glycoluril. Biochemical and Biophysical Research Communications 80, 849–57.
Kelly, C. (1987). Molecular studies of schistosome immunity. In The Biology of Schistosomes (ed. Rollinson, D. & Simpson, A. J. G.), pp. 265293. London and New York: Academic Press.
Knight, M., Simpson, A. J. G., Payares, G., Chaudhri, M. & Smithers, S. R. (1984). Cell-free synthesis of Schistosoma mansoni surface antigens: stage specificity of their expression. EMBO Journal 3, 213–19.
Knight, M., Kelly, C., Rodrigues, V., Yi, X., Wamachi, A., Smithers, S. R. & Simpson, A. J. G. (1989). A cDNA clone encoding part of the major Mr 25000 surface membrane antigen of adult Schistosoma mansoni. Parasitology Research 75, 281–6.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.
Omer Ali, P., Magee, A. I., Kelly, C. & Simpson, A. J. G. (1986). A major role for carbohydrates preferentially recognised by chronically infected mice in the determination of Schistosoma mansoni schistosomulum surface antigenicity. Journal of Immunology 137, 3601–7.
Omer Ali, P., Smithers, S. R., Bickle, Q., Phillips, S. M., Harn, D. & Simpson, A. J. G. (1988). Analysis of the anti-Schistosoma mansoni surface antibody response during murine infection and its potential contribution to protective immunity. Journal of Immunology 140, 3273–9.
Omer Ali, P., Hagan, P., Hackett, F., Smithers, S. R. & Simpson, A. J. G. (1989 a). Variable species and stage specificity of schistosomulum surface epitopes recognized by mice vaccinated with highly irradiated cercariae. Parasite Immunology 11, 301–18.
Omer Ali, P., Mansour, M., Woody, J. N., Smithers, S. R. & Simpson, A. J. G. (1989 b). Antibody to carbohydrate and polypeptide epitopes on the surface of schistosomula of Schistosoma mansoni in Egyptian patients with acute and chronic schistosomiasis. Parasitology 98, 417–24.
Payares, G., McLaren, D. J., Evans, W. H. & Smithers, S. R. (1985 a). Antigenicity and immunogenicity of the tegumental outer membrane of adult Schistosoma mansoni. Parasite Immunology 7, 4561.
Payares, G., Kelly, C., Smithers, S. R. & Evans, W. H. (1985 b). Evidence that the 32, 38 and 20 kilodalton surface antigens of schistosomula and schistosomes are a family of conserved proteins. Molecular and Biochemical Parasitology 17, 115–30.
Payares, G., McLaren, D. J., Evans, W. H. & Smithers, S. R. (1985 c). Changes in the surface antigen profile of Schistosoma mansoni during maturation from cercaria to adult worm. Parasitology 91, 8399.
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Fiqueredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: defined system for stepwise transformation of cercariae to schistosomula in vitro. Experimental Parasitology 36, 360–72.
Simpson, A. J. G., Hackett, F., Walker, T., Derossi, R. & Smithers, S. R. (1985). Antibody response against schistosomulum surface antigens and protective immunity following immunization with highly irradiated cercariae. Parasite Immunology 7, 133–52.
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695700.
Smithers, S. R., Hackett, F., Omer Ali, P. & Simpson, A. J. G. (1989). Protective immunization of mice against Schistosoma mansoni with purified adult worm surface membranes. Parasite Immunology 11, 257–67.
Von Lichtenberg, F. (1987). Consequences of infection with schistosomes. In The Biology of Schistosomes (ed. Rollinson, D. & Simpson, A. J. G.), pp. 185232. London and New York: Academic Press.

Keywords

Related content

Powered by UNSILO

Epitopes expressed on very low Mr Schistosoma mansoni adult tegumental antigens conform to a general pattern of life-cycle cross-reactivity

  • A. J. G. Simpson (a1), P. Hagan (a1), F. Hackett (a1), P. Omer Ali (a1) and S. R. Smithers (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.