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The effect of Giardia lamblia trophozoites on lipolysis in vitro

Published online by Cambridge University Press:  06 April 2009

P. Katelaris ,
F. Seow  and
M. Ngu
P. Katelaris
Affiliation:
Gastroenterology Unit, Concord Hospital, Sydney, Australia 2139
F. Seow
Affiliation:
Gastroenterology Unit, Concord Hospital, Sydney, Australia 2139
M. Ngu
Affiliation:
Gastroenterology Unit, Concord Hospital, Sydney, Australia 2139
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Extract

Infection with Giardia lamblia often causes only minor mucosal changes to the small intestine yet frank fat malabsorption may still occur. Some evidence suggests abtext-abstract pancreatic exocrine function in subjects with giardiasis although the mechanism and significance of this is unclear. Studies were conducted in vitro to determine the effect of G. lamblia trophozoites or culture filtrates from the organism on lipolysis of triglyceride by porcine pancreatic lipase. Live trophozoites significantly inhibited lipolysis. The degree of inhibition increased with longer duration of lipase exposure to trophozoites. Total amounts of enzyme inhibited were proportional to enzyme concentration, while the percentage inhibition was greatest at lowest concentration. At a lipase concentration of 1·7 i.u./ml, enzyme activity was reduced by 89·7% compared to controls after incubation for 4 h with trophozoites. The effect was abolished using killed, intact trophozoites. Culture filtrates of G. lamblia did not inhibit lipolysis. Specificity of the effect was suggested by the failure of another flagellate protozoan, Trichomonas vaginalis, to inhibit lipase. In this assay system the inhibition of lipolysis was not dependent on the bile salt concentration present. The impact of this effect in vivo remains to be determined but it may contribute to fat malabsorption in giardiasis.

Keywords

Giardia lamblialipase inhibitionpathogenesis of malabsorption
Type
Research Article
Information
Parasitology , Volume 103 , Issue 1 , August 1991 , pp. 35 - 39
Copyright
Copyright © Cambridge University Press 1991

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References

Borgstrom, B. & Erlanson, C. (1978). Interactions of serum albumin and other proteins with porcine pancreatic lipase. Gastroenterology 75, 382–6.CrossRefGoogle Scholar
Chawla, L. S., Sehgal, A. K., Broor, S. L., Verma, R. S. & Chhuttani, P. N. (1975). Tryptic activity in the duodenal aspirate following a standard test meal in giardiasis. Scandinavian Journal of Gastroenterology 10, 445–7.CrossRefGoogle ScholarPubMed
Das, S., Reiner, D. S., Zenian, J., Hogan, D. L., Koss, M. A., Wang, C.-S. & Gillin, F. D. (1988). Killing of Giardia lamblia trophozoites by human intestinal fluid in vitro. Journal of Infectious Diseases 157, 1257–60.CrossRefGoogle ScholarPubMed
Gillon, J. (1984). Giardiasis: review of epidemiology, pathogenetic mechanisms and host responses. Quarterly Journal of Medicine 209, 29–39.Google Scholar
Gupta, P. K. & Mehta, S. (1973). Giardiasis in children: a study of pancreatic functions. Indian Journal of Medical Research 61, 743–8.Google Scholar
Halliday, C. E. W., Clark, C. & Farthing, M. J. G. (1988). Giardia–bile salt interactions in vitro and in vivo. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 428–32.CrossRefGoogle ScholarPubMed
Hartong, W. A., Gourley, W. K. & Arvanitakis, C. (1979). Giardiasis: clinical spectrum and functional–structural abtext-abstractities of the small intestine mucosa. Gastroenterology 77, 61–9.Google Scholar
Jarroll, E. L., Muller, P. J., Meyer, E. A. & Morse, S. A. (1981). Lipid and carbohydrate metabolism of Giardia lamblia. Molecular and Biochemical Parasitology 2, 187–96.CrossRefGoogle ScholarPubMed
Keister, D. B. (1983). Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 487–8.CrossRefGoogle ScholarPubMed
Mashige, F., Tanaka, N., Maki, A., Kamei, S. & Yamanaka, M. (1978). Direct spectrophotometry of total bile acids in serum. Clinical Chemistry 27, 1352–6.CrossRefGoogle Scholar
Nash, T. E., Gillin, F. D. & Smith, P. D. (1983). Excretory–secretory products of Giardia lamblia. Journal of Immunology 131, 2004–10.CrossRefGoogle ScholarPubMed
Okada, M., Fuchigami, T., Ri, S., Kohrogi, N. & Omae, T. (1983). The BTPABA pancreatic function test in giardiasis. Postgraduate Medical Journal 59, 79–82.CrossRefGoogle ScholarPubMed
Owen, R. L., Nemanic, P. & Stevens, D. P. (1979). Ultrastructural observations on giardiasis in a murine model. Gastroenterology 76, 757–69.CrossRefGoogle ScholarPubMed
Philip, A., Carter-Scott, P. & Rogers, C. (1987). An agar culture technique to quantitate Trichomonas vaginalis from women. Journal of Infectious Diseases 155, 304–8.CrossRefGoogle ScholarPubMed
Smith, P. D., Horsburgh, C. R. & Brown, W. R. (1981). In vitro studies on bile acid deconjugation and lipolysis inhibition by Giardia lamblia. Digestive Diseases and Sciences 26, 700–4.CrossRefGoogle ScholarPubMed
Tandon, B. N., Tandon, R. K., Satpathy, B. K. & Shriniwas, . (1977). Mechanism of malabsorption in giardiasis: a study of bacterial flora and bile salt deconjugation in upper jejunum. Gut 18, 176–81.CrossRefGoogle ScholarPubMed
Tietz, N. W. & Fiereck, E. A. (1966). A specific method for serum lipase determination. Clinica Chimica Acta 13, 352.CrossRefGoogle ScholarPubMed
Tomkins, A. M., Drasar, B. S., Bradley, A. K. & Williamson, W. A. (1978). Bacterial colonisation of jejunal mucosa in giardiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 33–6.Google Scholar
Veghelyi, P. (1937). Celiac disease imitated by giardiasis. American Journal of Diseases of Children 57, 894–9.Google Scholar