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The effect of continuous doses of Trichostrongylus colubriformis larvae on the intestinal mucosa of sheep and on liver vitamin A concentration

Published online by Cambridge University Press:  06 April 2009

R. L. Coop  and
K. W. Angus
R. L. Coop
Affiliation:
Departments of Parasitology and Pathology, Moredun Research Institute, Edinburgh, Scotland
K. W. Angus
Affiliation:
Departments of Parasitology and Pathology, Moredun Research Institute, Edinburgh, Scotland
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Extract

Eight 7-month-old sheep were dosed continuously with Trichostrongylus colubriformis larvae and 4 sheep were killed at 5 and 10 weeks from the commencement of infection. Flattening of the mucosa and villous atrophy were commonly present at slaughter, and parasites were often found in superficial channels parallel with the luminal surface. At 10 weeks the mucosa was thickened and highly cellular. Leucine aminopeptidase, alkaline phosphatase and maltase activity were reduced in the proximal third of the small intestine. Liver vitamin A was reduced in 3 of 4 infected sheep at 10 weeks, but serum vitamin A levels were comparable with those of worm-free control sheep throughout the experiment.

Type
Research Article
Information
Parasitology , Volume 70 , Issue 1 , February 1975 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 1975

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References

Ames, S. R., Risley, H. A., & Harris, P. L., (1954). Simplified procedure for extraction and determination of vitamin A in liver. Analytical Chemistry 26, 1378–81.CrossRefGoogle Scholar
Bancroft, J. D., (1967). An Introduction to Histochemical Technique, p. 188. London: Butterworth.Google Scholar
Barker, I. K., (1973). Scanning electron microscopy of the duodenal mucosa of lambs infected with Trichostrongylus colubriformis. Parasitology 67, 307–14.CrossRefGoogle ScholarPubMed
Bloom, G., & Kelly, J. W., (1960). The copper phthalocyanin dye ‘Astrablue’ and its staining properties, especially the staining of mast cells. Histochimie 2, 48–57.CrossRefGoogle Scholar
Coop, R. L., Angus, K. W., & Mapes, C. J., (1973). The effect of large doses of Nematodirus battus on the histology and biochemistry of the small intestine of lambs. International Journal for Parasitology 3, 349–61.CrossRefGoogle ScholarPubMed
Croft, D. N., Loehry, C. A., & Creamer, B., (1968). Small bowel cell-loss and weight loss in the coeliac syndrome. Lancet 2, 68–70.CrossRefGoogle Scholar
Cuthbertson, D., & Smith, J. A. B., (1969). Nutrition of animals of agricultural importance. 2. Assessment of and factors affecting requirements of farm livestock. International Encyclopaedia of Food and Nutrition, vol. 17 (ed. Cuthbertson, D.), pp. 883–920. Oxford: Pergamon Press.Google Scholar
Dahlqvist, A., (1964). Method for assay of intestinal disaccharidases. Analytical Biochemistry 7, 18–25.CrossRefGoogle ScholarPubMed
Eveleth, D. F., Goldsby, A. I., Bolin, F. M., & Bolin, D. W., (1953). Effect of parasitism on conversion of carotene into vitamin A by sheep. A preliminary report. Veterinary Medicine 48, 441–2.Google Scholar
Fitzsimmons, W. M., (1966). Experimental Trichostrongylus colubriformis infection in the goat. Research in Veterinary Science 7, 101–11.CrossRefGoogle ScholarPubMed
Fitzsimmons, W. M., Harness, E., & Sellwood, S. A., (1968). Experimental Trichostrongylus colubriformis infection in adult goats. Research in Veterinary Science 9, 237–47.CrossRefGoogle Scholar
Gallagher, N. D., Playoust, M. R., & Symons, L. E. A., (1971). Mechanism of fat malabsorption in rats infected with Nippostrongylus brasiliensis. Gut 12, 1007–10.CrossRefGoogle ScholarPubMed
Gardiner, M. R., (1966). Pathological changes and vitamin B12 metabolism in sheep parasitised by Haemonchus contortus, Ostertagia spp. and Trichostrongylus colubriformis. Journal of Helminthology 40, 63–76.CrossRefGoogle ScholarPubMed
Green, M. N., Tsou, K. C, Bressler, R., & Seligman, A. M., (1955). The colorimetric determination of leucine aminopeptidase activity with L-leucyl-β-naphthylamide hydrochloride. Archives of Biochemistry and Biophysics 57, 458–74.CrossRefGoogle ScholarPubMed
Herlich, H., (1957). Preliminary observations on calves experimentally infected with Trichostrongylus colubriformis. Proceedings of the Helminthological Society of Washington 24, 139–40.Google Scholar
Kimble, M. S., (1939). The photocolorimetric determination of vitamin A and carotene in human plasma. Journal of Laboratory and Clinical Medicine 24, 1055–65.Google Scholar
Kvitkin, Yu. P., Smirnov, A. P., & Efimova, M. S., (1968). Nematodirus as competitive uses of carotene in lambs. Veterinariya 45, 42.Google Scholar
Mapes, C. J., & Coop, R. L., (1972). The development of single infections of Nematodirus battus in lambs. Parasitology 64, 197–216.CrossRefGoogle ScholarPubMed
Miller, H. R. P., & Jarrett, W. F. H., (1971). Immune reactions in mucous membranes. I. Intestinal mast cell response during helminth expulsion in the rat. Immunology 20, 277–88.Google ScholarPubMed
Padykula, H. A., Strauss, E. W., Ladman, A. J., & Gardner, F. H., (1961). A morphologic and histochemical analysis of the human jejunal epithelium in nontropical sprue. Gastroenterology 40, 735–65.CrossRefGoogle ScholarPubMed
Salomon, L. L., James, J., & Weaver, P. R., (1964). Assay of phosphatase activity by direct spectrophotometric determination of phenolate ion. Analytical Chemistry 36, 1162–4.CrossRefGoogle Scholar
Shiner, M., (1967). Intestinal absorption — Ultrastructure of jejunal surface epithelium in untreated idiopathic steatorrhoea. British Medical Bulletin 23, 223–5.CrossRefGoogle Scholar
Swanson, V. L., & Thomassen, R. W., (1965). Pathology of the jejunal mucosa in tropical sprue. American Journal of Pathology 46, 511–52.Google ScholarPubMed
Symons, L. E. A., (1965). Kinetics of the epithelial cells, and morphology of villi and crypts in the jejunum of the rat infected by the nematode Nippostrongylus brasiliensis. Gastroenterology 49, 158–68.CrossRefGoogle ScholarPubMed
Symons, L. E. A., (1969). Pathology of gastrointestinal helminthiases. International Review of Tropical Medicine 3, 49–100.Google ScholarPubMed
Symons, L. E. A., & Fairbairn, D., (1963). Biochemical pathology of the rat jejunum parasitized by the nematode Nippostrongylus brasiliensis. Experimental Parasitology 13, 284–304.CrossRefGoogle ScholarPubMed
Symons, L. E. A., & Jones, W. O., (1970). Nematospiroides dubius, Nippostrongylus brasiliensis and Trichostrongylus colubriformis: Protein digestion in infected mammals. Experimental Parasitology 27, 496–506.CrossRefGoogle ScholarPubMed
Symons, L. E. A., Gibbins, J. R., & Jones, W. O., (1971). Jejunal malabsorption in the rat infected by the nematode Nippostrongylus brasiliensis. International Journal for Parasitology 1, 179–87.CrossRefGoogle ScholarPubMed
Townley, R. R. W., Cass, M. H., & Anderson, C. M., (1964). Small intestinal mucosal patterns of coeliac disease and idiopathic steatorrhoea seen in other situations. Gut 5, 51–5.CrossRefGoogle ScholarPubMed
Yudkin, S., (1941). Estimation of vitamin A and carotene in human blood. Biochemical Journal 35, 551–6.CrossRefGoogle ScholarPubMed