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Differential targeting of dense granule proteins in the parasitophorous vacuole of Toxoplasma gondii

  • A. Achbarou (a1), O. Mercereau-Puijalon (a2), A. Sadak (a1), B. Fortier (a1), M. A. Leriche (a1), D. Camus (a1) and J. F. Dubremetz (a1)...

Extract

The biosynthesis and fate of 4 different dense granule proteins of Toxoplasma gondii were studied with 3 monoclonal antibodies raised against tachyzoites and 1 polyclonal antibody raised against a recombinant protein. These proteins have the following molecular weights: 27 kDa (GRA 1), 28 kDa (GRA 2), 30 kDa (GRA 3) and 40 kDa (GRA 4). All four proteins were found in dense granules by immunoelectron microscopy; in T. gondii-infected cells, they were found in the vacuolar network but, in addition, GRA 3 was also detected on the parasitophorous vacuole membrane. Therefore, dense granule contents undergo differential targeting when exocytosed in the parasitophorous vacuole. Metabolic labelling and immunoprecipitation showed that GRA 2 and GRA 3 were processed from lower molecular weight precursors, and that GRA 2 and GRA 4 incorporated [3H] glucosamine and are thus likely to be glycosylated.

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Keywords

Differential targeting of dense granule proteins in the parasitophorous vacuole of Toxoplasma gondii

  • A. Achbarou (a1), O. Mercereau-Puijalon (a2), A. Sadak (a1), B. Fortier (a1), M. A. Leriche (a1), D. Camus (a1) and J. F. Dubremetz (a1)...

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