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Detection of multiple infections by Monocystis strains in a single earthworm host using ribosomal internal transcribed spacer sequence variation

Published online by Cambridge University Press:  20 August 2009

T. P. VELAVAN ,
H. SCHULENBURG  and
N. K. MICHIELS
T. P. VELAVAN*
Affiliation:
Department of Animal Evolutionary Ecology, University of Tübingen, Germany Institute for Tropical Medicine, Department of Parasitology, Tübingen, Germany
H. SCHULENBURG
Affiliation:
Department of Animal Evolutionary Ecology, University of Tübingen, Germany Zoological Institute, Christian Albrechts University, Kiel, Germany
N. K. MICHIELS
Affiliation:
Department of Animal Evolutionary Ecology, University of Tübingen, Germany
*
*Corresponding author: Department of Animal Evolutionary Ecology, Zoological Institute, University of Tübingen, Auf der Morgenstelle 28 E, 72076 Tübingen, Germany. Tel: +49 7071 2974841. Fax: +49 7071 295634. E-mail: velavanp@yahoo.com
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Summary

Monocystis sp. are sporocyst-forming apicomplexan parasites common in seminal vesicles of the earthworm Lumbricus terrestris where they may account for temporary castration. This study describes the internal transcribed spacer (ITS) region of the ribosomal cistron of Monocystis sp. This region, including ITS-1, the 5·8S ribosomal RNA gene, and ITS-2, was PCR amplified, cloned, and sequenced for Monocystis sp. isolated from the seminal vesicles of several wild-caught L. terrestris. Our analysis revealed substantial polymorphisms, also within single host organisms, indicating intra-host diversity of parasites. These genetic markers are the first that allow distinction of Monocystis sp. genotypes, opening new avenues for the study of parasite diversity within and between hosts.

Keywords

Monocystis spribosomal RNApolymerase chain reaction
Type
Research Article
Information
Parasitology , Volume 137 , Issue 1 , January 2010 , pp. 45 - 51
Copyright
Copyright © Cambridge University Press 2009

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