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Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis

  • ALESSANDRA L. MORASSUTTI (a1), LISA N. RASCOE (a2), SUKWAN HANDALI (a2), ALEXANDRE J. DA SILVA (a3), PATRICIA P. WILKINS (a2) and CARLOS GRAEFF-TEIXEIRA (a1)...

Summary

The primary causative agent of eosinophilic meningoencephalitis (EoM) in endemic regions is the nematode Angiostrongylus cantonensis. The occurrence of EoM was previously restricted to countries in Southeast Asia and the Pacific Islands; however, more recently, it has been reported from other regions, including Brazil. The commonly used diagnosis is detection of specific antibody reactivity to the 31 kDa antigen, which is derived from female worm somatic extracts. Here we report the occurrence of cross-reactivity to this antigen in sera from other parasitic infections, especially those that may cause EoM, such as gnathostomiasis, toxocariasis, hydatidosis and strongyloidiasis. We also demonstrated that the cross-reactivity, in part, is dependent of the concentration of antigen used in Western blot assays. We discuss the importance of these findings on the interpretation of this test.

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Corresponding author

* Corresponding author: Instituto de Pesquisas Biomédicas da PUCRS, Avenida Ipiranga 6690, 2 andar, Sala 20, CEP: 90690-900 Porto Alegre RS, Brazil. E-mail: almorassutti@gmail.com

References

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Aguiar Prieto, P. H., Pascual Gispert, J., Dumenigo, B., Perera De Puga, G. and Gálvez Oviedo, M. D. (1981). Angiostrongylus cantonensis. Intermediate hosts in the 2 Havana provinces. Revista Cubana de Medicina Tropical 33, 173177.
de Savigny, D. H., Voller, A. and Woodruff, A. W. (1979). Toxocariasis: serological diagnosis by enzyme immunoassay. Journal of Clinical Pathology 32(3), 284288.
Eamsobhana, P., Tungtrongchitr, A., Wanachiwanawin, D., Yong, H. S. and Mak, J. W. (1998). Characterization of 31 kda specific Antigen from Parastrongylus cantonensis (Nematoda: Metastrongylidae). Journal of International Medical Research 2(1), 912.
Eamsobhana, P., Yoolek, A. and Suvouttho, S. (2001). Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels. Southeast Asian Journal of Tropical Medicine and Public Health 32, 308313.
Espírito-Santo, M. C., Pinto, P. L., Mota, D. J. and Gryschek, R. C. (2013). The first case of Angiostrongylus cantonensis eosinophilic meningitis diagnosed in the city of São Paulo, Brazil. Revista do Instituto de Medicina Tropical de São Paulo 55, 129132.
Graeff-Teixeira, C., Da Silva, A. C. and Yoshimura, K. (2009). Update on eosinophilic meningoencephalitis and its clinical relevance. Clinical and Microbiology Reviews 22, 322348. Table of Contents.
Kim, D. Y., Stewart, T. B., Bauer, R. W. and Mitchell, M. (2002). Parastrongylus (=Angiostrongylus) cantonensis now endemic in Louisiana wildlife. Journal for Parasitology 88, 10241026.
Jiang, L., Xu, X. N., Li, X., Xue, H. C. and Feng, Z. (2004). Identification of the immunodominant regions of the Em18 antigen and improved serodiagnostic specificity for alveolar echinococcosis. Parasite Immunology 10, 377385.
Jin, E., Ma, D., Liang, Y., Ji, A. and Gan, S. (2005). MRI findings of eosinophilic myelomeningoencephalitis due to Angiostrongylus cantonensis. Clinical Radiology 60, 242250.
Jin, E. H., Ma, Q., Ma, D. Q., He, W., Ji, A. P. and Yin, C. H. (2008). Magnetic resonance imaging of eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis following eating freshwater snails. Chinese Medical Jounal 121, 6772.
Lima, A. R., Mesquita, S. D., Santos, S. S., Aquino, E. R., Rosa, L. A. R., Duarte, F. S., Teixeira, A. O., Costa, Z. R. and Ferreira, M. L. (2009). Alicata disease: neuroinfestation by Angiostrongylus cantonensis in Recife, Pernambuco, Brazil. Arquivos de Neuro-Psiquiatria 67, 10931096.
Lindo, J. F., Escoffery, C. T., Reid, B., Codrington, G., Cunningham-Myrie, C. and Eberhard, M. L. (2004). Fatal autochthonous eosinophilic meningitis in a Jamaican child caused by Angiostrongylus cantonensis . American Journal of Tropical Medicine and Hygiene 70, 425428.
Morassutti, A. L., Levert, K., Perelygin, A., Da Silva, A. J., Wilkins, P. and Graeff-Teixeira, C. (2012). The 31-kDa antigen of Angiostrongylus cantonensis comprises distinct antigenic glycoproteins. Vector Borne and Zoonotic Diseases 12, 961968.
Morassutti, A. L., Perelygin, A., Levert, K., Lin, S. C., Lee, Y. M., Da Silva, A. J., Wilkins, P. P. and Graeff-Teixeira, C. (2013). Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report. Hawai'i Journal of Medicine & Public Health 72, 5862.
Morassutti Al Thiengo, Sc, Fernandez, M., Sawanyawisuth, K. and Graeff-Teixeira, C. (2014). Eosinophilic meningitis caused by Angiostrongylus cantonensis: an emergent disease in Brazil. Memorias do Instituto Oswaldo Cruz 109(4), 399407.
Nuamtanong, S. (1996). The evaluation of the 29 and 31 kDa antigens in female A. cantonensis for serodiagnosis of human angiostrongyliasis. Southeast Asian Journal of Tropical Medicine and Public Health 27, 291296.
Qvarnstrom, Y., Xayavong, M., da Silva, A. C., Park, S. Y., Whelen, A. C., Calimlim, P. S., Sciulli, R. H., Honda, S. A., Higa, K., Kitsutani, P., Chea, N., Heng, S., Johnson, S., Graeff-Teixeira, C., Fox, L. M. and da Silva, A. J. (2016). Real-Time polymerase chain reaction detection of Angiostrongylus cantonensis DNA in cerebrospinal fluid from patients with eosinophilic meningitis. American Journal of Tropical Medicine and Hygiene 94(1), 176181. doi: 10.4269/ajtmh.15–0146.
Sawanyawisuth, K., Takahashi, K., Hoshuyama, T., Senthong, V., Limpawattana, P., Intapan, P. M., Wilson, D., Tiamkao, S., Jitpimolmard, S. and Chotmongkol, V. (2009). Clinical factors predictive of encephalitis caused by Angiostrongylus cantonensis . American Journal of Tropical Medicine and Hygiene 81, 698701.
Slom, T. J., Cortese, M. M., Gerber, S. I., Jones, R. C., Holtz, T. H., Lopez, A. S., Zambrano, C. H., Sufit, R. L., Sakolvaree, Y., Chaicumpa, W., Herwaldt, B. L. and Johnson, S. (2002). An outbreak of eosinophilic meningitis caused by Angiostrongylus cantonensis in travelers returning from the Caribbean. New England Journal of Medicine 346, 668675.
Veríssimo, C. M., Morassutti, A. L., von Itzstein, M., Sutov, G., Hartley-Tassell, L., McAtamney, S., Dell, A., Haslam, S. M. and Graeff-Teixeira, C. (2016). Characterization of the N-glycans of female Angiostrongylus cantonensis worms. Experimental Parasitology 166, 137143. doi: 10.1016/j.exppara.2016.04.012
Wang, Q. P., Lai, D. H., Zhu, X. Q., Chen, X. G. and Lun, Z. R. (2008). Human angiostrongyliasis. Lancet Infectious Diseases 8, 621630.

Keywords

Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis

  • ALESSANDRA L. MORASSUTTI (a1), LISA N. RASCOE (a2), SUKWAN HANDALI (a2), ALEXANDRE J. DA SILVA (a3), PATRICIA P. WILKINS (a2) and CARLOS GRAEFF-TEIXEIRA (a1)...

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