Hostname: page-component-848d4c4894-ttngx Total loading time: 0 Render date: 2024-05-23T12:25:30.129Z Has data issue: false hasContentIssue false
  • English
  • Français

Correlation between the morphology and infectivity of Theileria lawrencei developing in the tick Rhipicephalus appendiculatus

Published online by Cambridge University Press:  06 April 2009

A. S. Young ,
R. E. Purnell ,
C. D. Kimber  and
R. C. Payne
A. S. Young
Affiliation:
Immunological Research on Tick-borne Cattle Diseases and Tick Control Project, East African Veterinary Research Organization, P.O. Box 32, Kikuyu, Kenya
R. E. Purnell
Affiliation:
Immunological Research on Tick-borne Cattle Diseases and Tick Control Project, East African Veterinary Research Organization, P.O. Box 32, Kikuyu, Kenya
C. D. Kimber
Affiliation:
Immunological Research on Tick-borne Cattle Diseases and Tick Control Project, East African Veterinary Research Organization, P.O. Box 32, Kikuyu, Kenya
R. C. Payne
Affiliation:
Immunological Research on Tick-borne Cattle Diseases and Tick Control Project, East African Veterinary Research Organization, P.O. Box 32, Kikuyu, Kenya
Get access Rights & Permissions [Opens in a new window]

Extract

Adult Rhipicephalus appendiculatus ticks, infected as nymphs by feeding them on an African buffalo harbouring Theileria lawrencei, were applied to the ears of rabbits.

Equal numbers of the feeding ticks were removed daily from rabbits and either dissected and processed so that parasites in their salivary glands could be examined morphologically and classified, or ground in Eagle's Minimal Essential Medium, to harvest parasites from the ticks in the resultant supernatant fluid. The infectivity of the supernatant fluid was tested by inoculation of aliquots into groups of susceptible cattle.

It was found that supernatant fluids produced from day 0 to day 2 fed ticks were uninfective to cattle but those produced from 3 to 9 days were infective. The most infective supernatant fluid was produced from 6 day fed ticks.

The reactions resulting in cattle were correlated with the number and morphology of parasites in sections of salivary glands and it was found that mature parasites coincided with infectivity of the supernatant fluid to cattle.

From the reactions in cattle inoculated with supernatant fluids from 5 and 6 day fed ticks it was concluded that this method of harvesting parasites was efficient.

Examination of the salivary glands of a representative sample of a T. lawrencei infected tick batch is a potential means of screening material for T. lawrencei stabilates.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 1 , August 1975 , pp. 27 - 34
Copyright
Copyright © Cambridge University Press 1975

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bailey, K. P. (1960). Notes on the rearing of Rhipicephalus appendiculatus and their infection with Theileria parva for experimental transmission. Bulletin of Epizootic Diseases of Africa 8, 33–43.Google Scholar
Barnett, S. F. & Brocklesby, D. W. (1966). The passage of ‘Theileria lawrencei (Kenya)’ through cattle. British Veterinary Journal 122, 396–409.Google Scholar
Burridge, M. J. & Kimber, C. D. (1972). The indirect fluorescent antibody test for experimental East Coast fever (Theileria parva infection of cattle). Evaluation of a cell culture schizont antigen. Research in Veterinary Science 13, 451–5.CrossRefGoogle ScholarPubMed
Burridge, M. J., Young, A. S., Stago, D. A., Kanhai, G. K. & Kimber, C. D. (1974). Theileria lawrencei infection of cattle and the Mrican buffalo: Evaluation of a buffalo derived cell culture schizont antigen for the indirect fluorescent antibody test. Research in Veterinary Science 16, 285–9.CrossRefGoogle Scholar
Cuningham, M. P., Brown, C. G. D., Burridge, M. J. & Purnell, R. E. (1973). Cryopreservation of the infective particles of Theileria pairva. International Journal of Parasitology 3, 583–7.CrossRefGoogle Scholar
Cunningham, M. P., Brown, C. G. D., Burridge, M. J., Musoke, A. J., Purnell, R. E., Radley, D. E. & Sempebwa, C. (1974). East Coast Fever: Titration in cattle of suspensions of Theileria parva derived from ticks. British Veterinary Journal 130, 336–45.CrossRefGoogle ScholarPubMed
Jarrett, W. F. H., Crighton, G. W. & Pirie, H. M. (1969). Theileria pearva: Kinetics of replication. Experimental Parasitology 24, 9–25.CrossRefGoogle ScholarPubMed
Purnell, R. E. & Joyner, L. P. (1968). The development of Theileria parva in the salivary glands of the tick Rhipicephalus appendiculatus. Parasitology 58, 725–32.CrossRefGoogle ScholarPubMed
Purnell, R. E., Brown, C. G. D., Cunningham, M. P., Burridge, M. J., Kirimi, I. M. & Ledger, M. A. (1973). East Coast fever: Correlation between the morphology and infectivity of Theileria parva developing in its tick vector. Parasitology 66, 539–44.CrossRefGoogle ScholarPubMed
Purnell, R. E., Young, A. S., Brown, C. G. D., Burridge, M. J. & Payne, R. C. (1974). Comparative infectivity of stabilates of Theileria lawrencei (Serengeti) derived from adult and nymphal ticks. Journal of Comparative Pathology 84, 533–7.CrossRefGoogle ScholarPubMed
Radley, D. E., Brown, C. G. D., Burridge, M. J., Cunnengham, M. P., Peirce, M. A. & Purnell, R. E. (1974). East Coast fever: Quantitative studies of Theileria parva in cattle. Experimental Parasitology 36, 278–87.Google Scholar
Stagg, D. A., Brown, C. G. D., Crawford, J. G., Kanhai, G. K. & Young, A. S. (1974). In vitro cultivation of Theileria lawrencei-infected lymphoblastoid cell lines derived from a buffalo (Syncerus caffer). Research in Veterinary Science 16, 125–7.Google Scholar
Young, A. S., Branagan, D., Brown, C. G. D., Burridge, M. J., Cunningham, M. P. & Purnell, R. E. (1973). Preliminary observations on a theilerial species pathogenic to cattle isolated from buffalo (Syncerus caffer) in Tanzania. British Veterinary Journal 129, 382–9.Google Scholar
Young, A. S. & Purnell, R. E. (1973). Transmission of Theileria lawrencei (Serengeti) by the ixodid tick Rhipicephalus appendiculatus. Tropical Animal Health & Production 5, 146–52.Google Scholar