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Comparison of immune responses between high and low responder strains of mice in the concomitant immunity and vaccine models of resistance to Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

Stephanie L. James  and
A. W. Cheever
Stephanie L. James
Affiliation:
Departments of Medicine and Microbiology, The George Washington University Medical Center, Washington, D.C. 20037
A. W. Cheever
Affiliation:
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20205
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Extract

Mice of the inbred P strain fail to develop significant resistance to challenge Schistosoma mansoni infection at 6 weeks after either low-grade primary infection or vaccination with attenuated homologous parasites, in contrast to other strains such as C57B1/6, and thus provide a model for comparison of potential immune resistance mechanisms in low versus high responder animals. In this study, the antigen-specific cellular responses found to correlate with resistance in these strains were delayed cutaneous hypersensitivity, production of macrophage activating lymphokine and macrophage larvicidal activity, all of which were greater in infected or vaccinated C57B1/6 mice than in similarly immunized but non-resistant P mice. Humoral responses correlating with resistance were IgM reactivity to schistosomula surface antigens in both infected and vaccinated animals, as well as both IgM and IgG reactivity to soluble schistosome antigens in infected mice. Immune responses that showed no relationship with resistance included IgG reactivity to larval surface antigens and immediate hypersensitivity to soluble worm antigens. In infected mice, neither granuloma size nor extent of hepatic fibrosis correlated positively with resistance to challenge infection. Thus, similarities exist between patterns of resistance and immune response at this early time after immunization with either viable or attenuated parasites. These observations suggest that common immune effector mechanisms could be involved, with activated macrophages playing a central role in resistance.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 2 , October 1985 , pp. 301 - 315
Copyright
Copyright © Cambridge University Press 1985

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