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Changes in protein expression in the sheep abomasum following trickle infection with Teladorsagia circumcincta

  • ALAN D. PEMBERTON (a1), JEREMY K. BROWN (a1), NICKY M. CRAIG (a1), JUDITH PATE (a1), KEVIN McLEAN (a2), NEIL F. INGLIS (a2), DAVID KNOX (a2) and PAMELA A. KNIGHT (a1)...

Summary

Continual low-level exposure of sheep to the helminth Teladorsagia circumcincta elicits a temporary protective immunity, where factors in the immune abomasal mucosa prevent penetration of infective larvae, but which is essentially lost within 6 weeks of cessation of parasite challenge. Here, a proteomic approach was used to identify proteins that are differentially regulated in immune compared to naïve sheep, as potential key mediators of immunity. Six naïve sheep and 12 sheep trickle-infected with T. circumcincta were treated with anthelmintic, and the naïve (control) and 6 immune sheep were killed 7 days later. The remaining 6 sheep (immune waning) were killed 42 days after anthelmintic treatment. Abomasal tissue samples were subjected to 2D-gel electrophoresis and densitometric analysis. Selected spots (n=73) were identified by peptide mass fingerprinting and confirmatory Western blotting was carried out for 10 proteins. Spots selectively up-regulated in immune versus control, but not immune waning versus control sheep, included galectin-15 and thioredoxin, which were confirmed by Western blotting. In immune sheep, serum albumin was significantly down-regulated and albumin proteolytic cleavage fragments were increased compared to controls. Unexpectedly, albumin mRNA was relatively highly expressed in control mucosa, down-regulated in immune, and was immunolocalized to mucus-producing epithelial cells. Thus we have identified differential expression of a number of proteins following T. circumcincta trickle infection that may play a role in host protection and inhibition of parasite establishment.

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Corresponding author

*Corresponding author: The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK. E-mail: alan.pemberton@ed.ac.uk

References

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Supplementary materials

Pemberton Supplementary Table 1
Supplementary Table 1: Sheep abomasal proteins identified by peptide mass fingerprinting

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Changes in protein expression in the sheep abomasum following trickle infection with Teladorsagia circumcincta

  • ALAN D. PEMBERTON (a1), JEREMY K. BROWN (a1), NICKY M. CRAIG (a1), JUDITH PATE (a1), KEVIN McLEAN (a2), NEIL F. INGLIS (a2), DAVID KNOX (a2) and PAMELA A. KNIGHT (a1)...

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