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Changes in cuticular permeability associated with recovery from anhydrobiosis in the plant parasitic nematode, Ditylenchus dipsaci

Published online by Cambridge University Press:  06 April 2009

D. A. Wharton ,
C. M. Preston ,
J. Barrett  and
R. N. Perry
D. A. Wharton
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
C. M. Preston
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
J. Barrett
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
R. N. Perry*
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
*
3Department of Nematology and Entomology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ UK.
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Summary

The cuticular permeability of anhydrobiotic 4th-stage juveniles of Ditylenchus dipsaci decreases during rehydration, as indicated by staining with osmium tetroxide, the measurement of permeability coefficients by the rate of uptake of tritiated water from a HOH/C-inulin bathing solution and by an increased ability to slow down the rate of water loss and to survive subsequent desiccation. The initial decrease in permeability coefficient is rapid, suggesting a physical effect due to the rehydration of the cuticle, followed by a slower reduction over the subsequent 1–4 h which is dependent upon metabolic activity. The reestablishment of the permeability barrier is sensitive to metabolic inhibitors which affect enzyme activity and post-transcriptional protein synthesis. Sodium iodoacetamide was the only inhibitor which affected maintenance of the permeability barrier. An Arrhenius plot of changes in cuticular permeability with temperature indicated a sharp increase in permeability at 40–50°C and brief exposure to diethyl ether resulted in loss of the permeability barrier, suggesting that a superficial layer, probably the epicuticle, is responsible for controlling cuticular permeability.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 2 , October 1988 , pp. 317 - 330
Copyright
Copyright © Cambridge University Press 1988

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Footnotes

1

Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand.

2

Department of Microbiology, University College, Newport Rd, Cardiff CF2 1TA, UK.

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