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Carvacryl acetate nanoencapsulated with chitosan/chichá gum exhibits reduced toxicity in mice and decreases the fecal egg count of sheep infected with gastrointestinal nematodes

Published online by Cambridge University Press:  09 July 2021

Weibson P. P. André Open the ORCID record for Weibson P. P. André [Opens in a new window] ,
José R. P. Junior Open the ORCID record for José R. P. Junior [Opens in a new window] ,
Géssica S. Cavalcante Open the ORCID record for Géssica S. Cavalcante [Opens in a new window] ,
Wesley L. C. Ribeiro Open the ORCID record for Wesley L. C. Ribeiro [Opens in a new window] ,
José V. Araújo-Filho Open the ORCID record for José V. Araújo-Filho [Opens in a new window] ,
Selene M. Morais Open the ORCID record for Selene M. Morais [Opens in a new window] ,
Lorena M. B. Oliveira Open the ORCID record for Lorena M. B. Oliveira [Opens in a new window] ,
Flávia O. M. S. Abreu Open the ORCID record for Flávia O. M. S. Abreu [Opens in a new window]  and
Claudia M. L. Bevilaqua Open the ORCID record for Claudia M. L. Bevilaqua [Opens in a new window]
Weibson P. P. André
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
José R. P. Junior
Affiliation:
Laboratório de Química Analítica e Ambiental, Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará, Fortaleza, Brazil
Géssica S. Cavalcante
Affiliation:
Laboratório de Química de Produtos Naturais, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Wesley L. C. Ribeiro
Affiliation:
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Brazil
José V. Araújo-Filho
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Selene M. Morais
Affiliation:
Laboratório de Química de Produtos Naturais, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Lorena M. B. Oliveira
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
Flávia O. M. S. Abreu
Affiliation:
Laboratório de Química Analítica e Ambiental, Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará, Fortaleza, Brazil
Claudia M. L. Bevilaqua*
Affiliation:
Laboratório de Doenças Parasitarias, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, Brazil
*
Author for correspondence: Claudia M. L. Bevilaqua, E-mail: bevilaqua.uece@gmail.com
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Abstract

The nanoencapsulation of biocomposites with anthelmintic action has been proposed as an alternative for improving their efficiency. Thus, the current study aimed to evaluate the efficacy of carvacryl acetate nanoencapsulated with biopolymers (nCVA) in the control of sheep gastrointestinal nematodes. CVA was nanoencapsulated with chitosan/chichá gum and characterized in terms of its efficacy of encapsulation (EE), yield and zeta potential. The acute toxicity of nCVA was evaluated in mice. For the fecal egg count reduction test, 40 animals were divided into four groups (n = 10) and orally administered the following treatments: G1, 250 mg kg−1 CVA; G2, 250 mg kg−1 nCVA; G3, chitosan/chichá gum (negative control) and G4, 2.5 mg kg−1 monepantel (positive control). Feces were collected on days 0 and 16 posttreatment to determine the eggs per gram of feces (epg). The EE and yield of nCVA were 72.8 and 57.5%, respectively. The nanoparticles showed a size of 764.5 ± 302.5 nm, and the zeta potential at pH 3.2 was +22.0 mV. nCVA presented a 50% lethal dose (LD50) of 2609 mg kg−1. By 16 days posttreatment, CVA, nCVA and monepantel reduced the epg by 52.9.7, 71.5 and 98.7%, respectively, and the epg of sheep treated with nCVA differed from that of the negative control (P > 0.05) but did not differ from that of sheep treated with CVA. In conclusion, the nanoencapsulation of CVA reduced its toxicity, and nCVA showed anthelmintic activity.

Keywords

AcetylationbiopolymerscarvacrolHaemonchus contortustoxicity
Type
Research Article
Information
Parasitology , Volume 148 , Issue 13 , November 2021 , pp. 1560 - 1565
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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