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Babesia divergens infections in the Mongolian gerbil: characteristics of a human strain

Published online by Cambridge University Press:  06 April 2009

K. G. Liddell ,
S. B. Lucas  and
H. Williams
K. G. Liddell
Affiliation:
Department of Microbiology, Raigmore Hospital, Inverness IV2 3UJ, Scotland
S. B. Lucas
Affiliation:
Department of Geographical Pathology, St Thomas's Hospital Medical School, London SE1 7EH
H. Williams
Affiliation:
Department of Microbiology, Raigmore Hospital, Inverness IV2 3UJ, Scotland
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Summary

A strain of the cattle piroplasm Babesia divergens isolated from a fatal human infection was propagated in the Mongolian gerbil through 150 semi-continuous intraperitoneal passages. The infection was normally fatal; death, accompanied by profuse haemoglobinuria and debilitation, occurred as early as 44 h after intraperitoneal inoculation of heavily parasitized blood with precipitous drops in red blood cell and platelet counts. The average maximum parasitaemia achieved increased on continuous passage reaching 80% by the 150th stage. Twenty-four hours after infection erythrophagocytosis and splenic congestion were apparent by light and electron-microscopical examination and by 48 h hepatic necrosis, renal tubular damage with haemoglobin cast accumulation and ischaemic necrosis of ileal mucosa had developed. Gerbils were highly susceptible to small numbers of parasites when the inoculum was either fresh parasitized blood in high dilution or erythrocytes concentrated from animals showing minimal parasitaemia. Animals inoculated with parasites preserved in dimethyl sulphoxide at low temperatures usually developed fatal infections. However, occasionally animals suffered at most a low grade parasitaemia subsequent to recovery with parasite elimination. These animals were immune to further challenge, and no chronic infections developed. A. field strain of B. divergens isolated locally from a case of bovine redwater behaved similarly to the human strain on continuous passage in gerbils.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 2 , April 1981 , pp. 205 - 224
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Adam, K. M. G. & Blewett, D. A. (1974). Experimental infection of mice with Babesia divergens. Journal of Protozoology 21 (Suppl.), 448.Google Scholar
Allison, A. C., Christensen, J., Clark, I. A., Elford, B. C. & Eugui, E. M. (1979). The role of the spleen in protection against murine babesia infections. In The Role of the Spleen in the Immunology of Parasitic Diseases. Tropical Diseases Research Series No. 1, pp. 151–82. Basel: Schwabe and Company, AG.Google Scholar
Anderson, A. E., Cassaday, P. B. & Healy, G. R. (1974). Babesiosis in man — sixth documented case. American Journal of Clinical Pathology 62, 612–18.CrossRefGoogle ScholarPubMed
Benach, J. L., White, D. J. & McGovern, J. P. (1978). Babesiosis in Long Island. Host–parasite relationships of rodent- and human-derived Babesia microti isolates in hamsters. American Journal of Tropical Medicine and Hygiene 27, 1073–8.CrossRefGoogle ScholarPubMed
Benach, J. L., White, D. J., McGovern, J. P. & Jacovina, M. M. (1979). Immunological relationships of Long Island isolates of Babesia microti. American Journal of Tropical Medicine and Hygiene 28, 643–8.CrossRefGoogle ScholarPubMed
Brandt, F., Healy, G. R. & Welch, M. (1977). Human babesiosis: the isolation of Babesia microti in golden hamsters. Journal of Parasitology 63, 934–7.CrossRefGoogle ScholarPubMed
Callow, L. L., Mellors, L. T. & McGregor, W. (1979). Reduction in virulence of Babesia bovis due to rapid passage in splenectomized cattle. International Journal for Parasitology 9, 333–8.CrossRefGoogle ScholarPubMed
Canning, E. U., Killick-Kendrick, R. & Monk, J. B. (1976). Morphology of piroplasms in abnormal hosts and the identification of piroplasms of man. Journal of Tropical Medicine and Hygiene 79, 5–8.Google ScholarPubMed
Dalgliesh, R. J., Dimmock, C. K., Hill, M. W. M. & Mellors, L. T. (1976). Babesia argentina: disseminated intravascular coagulation in acute infections in splenectomised calves. Experimental Parasitology 40, 124–31.CrossRefGoogle Scholar
Dammin, G. J. (1978). Babesiosis. In Seminars in Infectious Disease, vol. 1 (ed. Weinstein, L. and Fields, B.) pp. 169–99. New York: Stratton Intercontinental Medical Book Corporation.Google Scholar
Dillon, W. G. & Glomski, C. A. (1975 a). The Mongolian gerbil: qualitative and quantitative aspects of the cellular blood picture. Laboratory Animals 9, 283–7.CrossRefGoogle ScholarPubMed
Dillon, W. G. & Glomski, C. A. (1975 b). Erythrocyte survival in the Mongolian gerbil. Journal of Nuclear Medicine 16, 682–4.Google ScholarPubMed
Entrican, J. H., Williams, H., Cook, I. A., Lancaster, W. M., Clark, J. C., Joyner, L. P. & Lewis, D. (1979 a). Babesiosis in man: a case from Scotland. British Medical Journal 2, 474.CrossRefGoogle Scholar
Entrican, J. H., Williams, H., Cook, I. A., Lancaster, W. M., Clark, J. C., Joyner, L. P. & Lewis, D. (1979 b). Babesiosis in man: report of a case from Scotland with observations on the infecting strain. Journal of Infection 1, 227–34.CrossRefGoogle Scholar
Gleason, N. N., Healy, G. R., Western, K. A., Benson, G. D. & Schultz, M. G. (1970). The ‘Gray’ strain of Babesia microti from a human case established in laboratory animals. Journal of Parasitology 56, 1256–7.CrossRefGoogle ScholarPubMed
Goodger, B. V. & Wright, I. G. (1977). Babesia bovis (argentina): observations of coagulation parameters, fibrinogen catabolism and fibrinolysis in intact and splenectomized cattle. Zeitschrift für Parasitenkunde 54, 9–27.CrossRefGoogle ScholarPubMed
Hussein, H. S. (1977 a). The pathology of Babesia hylomysci infection in mice. 1. Clinical signs and liver lesion. Journal of Comparative Pathology 87, 161–7.CrossRefGoogle Scholar
Hussein, H. S. (1977 b). The pathology of Babesia hylomysci infection in mice. II. Kidney lesion. Journal of Comparative Pathology 87, 169–75.CrossRefGoogle ScholarPubMed
Irvin, A. D. & Brocklesby, D. W. (1972). Attempts to transmit Babesia (Nuttallia) microti in laboratory mice by the tick Rhipicephalus appendiculatus. International Journal for Parasitology 2, 99–103.CrossRefGoogle ScholarPubMed
Irvin, A. D., Young, E. R. & Osborn, G. D. (1978). Attempts to infect T lymphocyte-deficient mice with Babesia species of cattle. Research in Vetrinary science 25, 245–6.CrossRefGoogle ScholarPubMed
Lathe, G. H. & Ruthven, C. R. (1958). Factors affecting the rate of coupling of bilirubin and conjugated bilirubin in the van de Bergh reaction. Journal of Clinical Pathology 11, 155–61.CrossRefGoogle Scholar
Lewis, D. & Williams, H. (1979). Infection of the Mongolian gerbil with the cattle piroplasm Babesia divergens. Nature, London 278, 170–1.CrossRefGoogle ScholarPubMed
Lykins, J. D., Ristic, M., Weisiger, R. M. & Huxsoll, D. L. (1975). Babesia microti: pathogenesis of parasite of human origin in the hamster. Experimental Parasitology 37, 388–97.CrossRefGoogle ScholarPubMed
Maegraith, B. G., Gilles, H. M., & Devakul, K. (1957). Pathological processes in Babesia canis infections. Zeitschrift für Tropenmedizin und Parasitologie 8, 485–514.Google ScholarPubMed
Malherbe, W. D. (1956). The manifestations and diagnosis of Babesia infections. Annals of the New York Academy of Sciences 64, 128–46.CrossRefGoogle Scholar
Malherbe, W. D. (1966). Clinico-pathological studies of Babesia canis infection in dogs. V. The influence of the infection on kidney function. Journal of the South African Veterinary Medical Association 37, 261–4.Google Scholar
Marston, J. H. (1976). The Mongolian gerbil. In The UFAW Handbook on the Care and Management of Laboratory Animals 5th ed., pp. 263–74. Edinburgh, London and New York: Churchill Livingstone.Google Scholar
Mays, A. JR. (1969). Baseline hematological and blood biochemical parameters of the Mongolian gerbil (Meriones unguiculatus). Laboratory Animal Care 19, 838–42.Google ScholarPubMed
Miller, L. H., Neva, F. A. & Gill, F. (1978). Failure of chloroquine in human babesiosis (Babesia microti). Case report and chemotherapeutic trial in hamsters. Annals of Internal Medicine 88, 200–2.CrossRefGoogle ScholarPubMed
Najarian, H. H. (1961). Haemobartonella in the Mongolian gerbil. Texas Reports on Biology and Medicine 19, 123–33.Google ScholarPubMed
Osorno, B. M., Vega, C., Ristic, M., Robles, C. & Ibarra, S. (1976). Isolation of Babesia spp. from asymptomatic human beings. Veterinary Parasitology 2, 111–20.CrossRefGoogle Scholar
Paget, G. E., Alcock, S. J. & Ryley, J. F. (1962). The pathology of Babesia rodhaini infections in mice. Journal of Pathology and Bacteriology 84, 218–20.CrossRefGoogle ScholarPubMed
Rabinovich, S. A., Voronina, L. K., Stepanova, N. I., Maruashvily, G. M., Bakradze, T. L., Odisharia, M. S. & Gvasalia, N. I. (1978). First dectection of human babesiosis in the U.S.S.R. and a short analysis of the cases described in the literature. Meditsinskaya Parazitologiya 47, 97–107.Google Scholar
Ristic, M. & Lewis, G. E. Jr (1976). Babesia in man and wild and laboratory-adapted mammals. In Parasitic Protozoa, vol. 4 (ed. Kreier, J. P.), pp. 53–76. New York, San Francisco and London: Academic Press.Google Scholar
Rogers, R. J. (1971). Observations on the pathology of Babesia argentina infections in cattle. Australian Veterinary Journal 47, 242–7.CrossRefGoogle ScholarPubMed
Rousselot, R. (1947). Parasites du sang de divers animaux de Ia région de Teheran. Archives of the Hessarek Institute 5, 62–72.Google Scholar
Rousselot, R. (1953). Notes de Parasitologie Tropicale. I. Parasites de Sang des Animaux. Paris: Vigot Frères.Google Scholar
Ruhren, R. (1965). Normal values for hemoglobin concentration and cellular elements in the blood of Mongolian gerbils. Laboratory Animal Care 15, 313–20.Google ScholarPubMed
Simpson, C. F. (1970). Electron microscopic comparison of Babesia spp. and hepatic changes in ponies and mice. American Journal of Veterinary Research 31, 1763–8.Google ScholarPubMed
Škrabalo, Z. & Deanovíc, Z. (1957). Piroplasmosis in man. Report on a case. Documenta de Medicina Geographica et Tropica 9, 11–16.Google ScholarPubMed
Smith, R. A., Termer, E. A. & Glomski, C. A. (1976). Erythrocyte basophilic stippling in the Mongolian gerbil. Laboratory Animals 10, 379–83.CrossRefGoogle ScholarPubMed
Spielman, A., Carleton, C. M., Piesman, J. & Corwin, M. D. (1979). Human babesiosis on Nantucket Island U.S.A.: description of the vector Ixodes (Ixodes) dammini, n.sp. (Acarina: Ixodidae). Journal of Medical Entomology 15, 218–34.CrossRefGoogle ScholarPubMed
Weiss, M. L. (1976). Plasmodium berghei: adaptation of a mouse-adapted strain to the Mongolian jird (Meriones unguiculatus); infectivity and immunogenicity. Experimental Parasitology 40, 103–11.CrossRefGoogle Scholar
Wightman, S. R., Pilitt, P. A. & Wagner, J. E. (1978). Dentostomella translucida in the Mongolian gerbil (Meriones unguiculatus). Laboratory Animals Science 28, 290–6.Google ScholarPubMed
Wright, I. G. (1972). Studies on the pathogenesis of Babesia argentina and Babesia bigemina infections in splenectomized calves. Zeitschrift für Parasitenkunde 39, 85–102.CrossRefGoogle Scholar
Wright, I. G. (1973). Observations on the haematology of experimentally induced Babesia argentina and B. bigemina infections in splenectomised calves. Research in Veterinary Science 14, 29–34.CrossRefGoogle Scholar
Wright, I. G. (1975). The probable role of Babesia argentina esterase in the in vitro activation of plasma prekallikrein. Veterinary Parasitology 1, 91–6.CrossRefGoogle Scholar
Wright, I. G. (1977). Kinin, kininogen and kininase levels during acute Babesia bovis infection of cattle. British Journal of Pharmacology 61, 567–72.CrossRefGoogle ScholarPubMed
Wright, I. G. & Kerr, J. D. (1974). The preferential invasion of young erythrocytes in acute Babesia bigemina infections of splenectomised calves. Zeitschrift für Parasitenkunde 43, 63–9.CrossRefGoogle ScholarPubMed