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Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

Published online by Cambridge University Press:  22 September 2020

Rayan Rubens da Silva Alves ,
João Gustavo Mendes Rodrigues ,
Andrea Teles-Reis ,
Ranielly Araújo Nogueira ,
Irlla Correia Lima Licá ,
Maria Gabriela Sampaio Lira ,
Raynara da Silva Alves ,
Nêuton Silva-Souza ,
Teresinha de Jesus Aguiar dos Santos Andrade  and
Guilherme Silva Miranda Open the ORCID record for Guilherme Silva Miranda [Opens in a new window]
Rayan Rubens da Silva Alves
Affiliation:
Departamento de Química e Biologia, Universidade Estadual do Maranhão, São Luís, MA, Brasil
João Gustavo Mendes Rodrigues
Affiliation:
Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
Andrea Teles-Reis
Affiliation:
Departamento de Química e Biologia, Universidade Estadual do Maranhão, São Luís, MA, Brasil
Ranielly Araújo Nogueira
Affiliation:
Departamento de Patologia, Universidade Federal do Maranhão, São Luís, MA, Brasil
Irlla Correia Lima Licá
Affiliation:
Departamento de Patologia, Universidade Federal do Maranhão, São Luís, MA, Brasil
Maria Gabriela Sampaio Lira
Affiliation:
Departamento de Patologia, Universidade Federal do Maranhão, São Luís, MA, Brasil
Raynara da Silva Alves
Affiliation:
Departamento de Química e Biologia, Universidade Estadual do Maranhão, São Luís, MA, Brasil
Nêuton Silva-Souza
Affiliation:
Departamento de Química e Biologia, Universidade Estadual do Maranhão, São Luís, MA, Brasil
Teresinha de Jesus Aguiar dos Santos Andrade
Affiliation:
Departamento de Educação, Instituto Federal de Educação, Ciência e Tecnologia do Maranhão, São Raimundo das Mangabeiras, MA, Brasil
Guilherme Silva Miranda*
Affiliation:
Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil Departamento de Educação, Instituto Federal de Educação, Ciência e Tecnologia do Maranhão, São Raimundo das Mangabeiras, MA, Brasil
*
Author for correspondence: Guilherme Silva Miranda, E-mail: mirandagsbio@gmail.com
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Abstract

New treatment strategies for schistosomiasis should be evaluated, since resistant strains to the only available drug, Praziquantel, have already been described. Thus, we demonstrated antiparasitic effects of ethanolic extracts of Jatropha gossypiifolia and Piper arboreum on cercariae and adult worms of Schistosoma mansoni. The bioassays were performed at 0–10 000 μg mL−1 concentration for 0–72 h. Adult worms were stained with carmine to assess external and internal damage. The chemical screening was performed using high-performance liquid chromatography. P. arboreum displayed the best cercaricidal effect, with a 100% reduction in viability in just 60 min. The extract of J. gossypiifolia was more effective against adult worms, with 100% viability reduction of male and female worms after 12 and 24 h, respectively. P. arboreum and J. gossypiifolia were equally effective in inhibiting the oviposition of S. mansoni (93% reduction) and causing damage to internal and external structures in adult worms. Flavonoids were identified in both the extracts and phenolic compounds and amides only in P. arboreum. Thus, for the first time, it was proven that ethanolic extracts of P. arboreum and J. gossypiifolia leaves are biologically active against cercariae and adult worms of S. mansoni in vitro.

Keywords

Morphological damageplant extractsschistosomiasissecondary metabolitestreatment
Type
Research Article
Information
Parasitology , Volume 147 , Issue 14 , December 2020 , pp. 1689 - 1699
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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