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Antigenic analysis of sequential erythrocytic stages of Plasmodium knowlesi

Published online by Cambridge University Press:  06 April 2009

J. A. Deans ,
E. D. Dennis  and
S. Cohen
J. A. Deans
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London SE1 9RT
E. D. Dennis
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London SE1 9RT
S. Cohen
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London SE1 9RT
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Summary

The antigenic composition of sequential erythrocytic stages of Plasmodium knowlesi has been compared by crossed immuno-electrophoresis using a pool of immune rhesus monkey antiserum. Eleven major parasite antigens have been identified; 9 are stage-independent, and 2 stage-dependent. Differences in the relative amount of the stage-independent antigens have been demonstrated and quantified. The distribution of antigens between parasites and schizont and infected red cell membranes has been examined. Only 6 of the 11 parasite antigens were exhibited by a schizont membrane preparation, all these antigens were also expressed by the intracellular parasite. Antigens exclusive to the schizont membrane were not demonstrated.

Type
Research Article
Information
Parasitology , Volume 77 , Issue 3 , December 1978 , pp. 333 - 344
Copyright
Copyright © Cambridge University Press 1978

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