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Antigen shedding from the surface of the infective stage larvae of Dirofilaria immitis

Published online by Cambridge University Press:  06 April 2009

M. S. Ibrahim ,
W. K. Tamashiro ,
D. A. Moraga  and
A. L. Scott
M. S. Ibrahim
Affiliation:
Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205
W. K. Tamashiro
Affiliation:
Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205
D. A. Moraga
Affiliation:
Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205
A. L. Scott
Affiliation:
Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205
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Summary

A qualitative and quantitative analysis was made of the release of surface-associated molecules from developing Dirofilaria immitis infective-stage larvae (L3). D. immitis L3s were labelled with 125I using an Iodogen catalysed reaction and either maintained in culture or placed in chambers that were implanted into Lewis rats. The larvae released 10–20% of the labelled material each day during the first 4 days of in vitro and in vivo development. The loss of surface-labelled peptides from developing larvae corresponded with an increase in the amount of trichloroacetic acid-precipitable radioactivity found in the culture medium. SDS—PAGE analysis of the labelled material showed that the same 35 and 6 kDa components found in larval extracts were shed into culture medium by the developing parasites. Metabolic labelling studies and experiments in which larvae were labelled after different times in culture indicated that, once released, the surfaceassociated molecules were not replaced, and that this net loss of surface peptides resulted in a reduction in the antigenic potential of the cuticular surface. Antibodies from both immunized rabbits and naturally infected dogs immunoprecipitated the 35 kDa component. In contrast, the 6 kDa molecule was not recognized by the antibodies in any of the sera tested. Shedding of surface peptides and reducing surface antigenicity may represent mechanisms by which D. immitis infective-stage larvae evade immune attack.

Keywords

Dirofilaria immitisinfective-stage larvaesurface-associated antigensantigen sheddingevasion of the immune response.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 1 , August 1989 , pp. 89 - 97
Copyright
Copyright © Cambridge University Press 1989

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