Hostname: page-component-848d4c4894-4rdrl Total loading time: 0 Render date: 2024-06-30T12:04:53.689Z Has data issue: false hasContentIssue false
  • English
  • Français

An in vitro investigation of dynamic processes occurring in the schistosome tegument, using compounds known to disrupt secretory processes

Published online by Cambridge University Press:  06 April 2009

R. A. Wilson  and
P. E. Barnes
R. A. Wilson
Affiliation:
Department of Biology, University of York, Heslington, York
P. E. Barnes
Affiliation:
Department of Biology, University of York, Heslington, York
Get access Rights & Permissions [Opens in a new window]

Extract

The structure of the schistosome tegument can be changed by in vitro incubation of worms in medium containing chemicals selected for their ability to disrupt secretion in other cell systems. The observed structural changes support the hypothesis that the function of the discoid granules is to maintain the integrity of the tegument ground substance. They further support the idea that the multilaminate vesicles and/or their contents are incorporated into or on to the worm surface as part of a mechanism of surface turnover. Vesicle life appears to be similar to that estimated for other trematodes and in cestodes. In addition the worm surface appears to be replaced more rapidly than the worm acquires and loses its disguise of host antigens. It is suggested that the surface channels of the tegument may be a variable feature, and that transfer of fluids between environment and interstitial spaces may occur across the tegument.

Type
Research Article
Information
Parasitology , Volume 68 , Issue 2 , April 1974 , pp. 259 - 270
Copyright
Copyright © Cambridge University Press 1974

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allison, A. C. (1972). Cell membranes and immune responses. In Functional Aspects of Parasite Surfaces. Symposia of the British Society for Parasitology 10 (eds. Taylor, A. E. R. and Muller, R.), pp. 93–107. Oxford and London: Blackwell.Google Scholar
Allison, A. C., Davies, P. & De Petris, S. (1971). Role of contractile microfliaments in macrophage movement and endocytosis. Nature, New Biology, London 232, 153–5.Google Scholar
Amsterdam, A., Ohad, I. & Schramm, M. (1969). Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle. Journal of Cell Biology 41, 753–73.CrossRefGoogle ScholarPubMed
Babad, H., Ben-Zvi, R., Bdolah, A. & Schramm, M. (1967). The mechanism of enzyme secretion by the cell. 4. Effects of inducers, substrates and inhibitors on amylase secretion by rat parotid slices. European Journal of Biochemistry 1, 96–101.CrossRefGoogle ScholarPubMed
Batzri, S., Amsterdam, A., Selinger, Z., Ohad, I. & Schramm, M. (1971). Epinephrine induced vacuole formation in parotid gland cells and its independence of the secretory process. Proceedings of the National Academy of Sciences 68, 121–3.CrossRefGoogle ScholarPubMed
Bennett, J., Beuding, E., Timms, A. R. & Engstrom, R. G. (1969). Occurrence and levels of 5-hydroxytryptamine in Schistosoma mansoni. Molecular Pharmacology 5, 542–5.Google ScholarPubMed
Caro, L. G. & Palade, G. E. (1964). Protein synthesis, storage and discharge in the pancreatic exocrine cell: an autoradiographic study. Journal of Cell Biology 20, 173–95.Google ScholarPubMed
Carter, S. B. (1972). The cytochalasins as research tools in cytology. Endeavour 31, 77–82.Google ScholarPubMed
Clegg, J. A., Smithers, S. H. & Terry, R. J. (1970). ‘Host’ antigens associated with schistosomes: observations on their attachment and their nature’. Parasitology 61, 87–94.CrossRefGoogle ScholarPubMed
Duguid, R. & Marsden, J. C. (1973). Effects of ouabain on muco-substance secretion in cell culture. Biochemical Society Transactions 1, 106–7.CrossRefGoogle Scholar
Gallagher, J. T. (1970). Studies related to the metabolism of mucosubstances. D. Phil. Thesis, University of York.Google Scholar
Ho, R-J., Jeanrenaud, B. & Renold, A. E. (1966). Ouabain-sensitive fatty acid release from isolated fat cells. Experientia 22, 86–7.CrossRefGoogle ScholarPubMed
Jamieson, J. D. & Palade, G. E. (1968). Intracellular transport of secretory proteins in the pancreatic exocrine cell. III. Dissociation of intracellular transport from protein synthesis. Journal of Cell Biology 39, 580–8.CrossRefGoogle Scholar
Jasper, D. K. & Bronk, J. R. (1968). Studies on the physiological and structural characteristics of rat intestinal mucosa. Mitochondrial structural changes during amino acid absorption. Journal of Cell Biology 38, 277–91.CrossRefGoogle ScholarPubMed
Lumsden, R. D. (1966). Cytological studies on the absorptive surfaces of cestodes. II. The synthesis and intracellular transport of protein in the strobilar integument of Hymenolepis diminuta. Zeitschrift für Parasitenkunde 28, 1–13.Google ScholarPubMed
Mansour, T. E. & Mansour, J. M. (1962). Effects of serotonin (5-hydroxytryptamine) and adenosine 3′, 5′-phosphate on phosphofructokinase from the liver fluke Fasciola hepatica. Journal of Biological Chemistry 237, 629–34.CrossRefGoogle Scholar
Morré, D. J. & Mollenhauer, H. H. (1974). The endomembrane concept. In Dynamic Aspects of Plant Ultrastructure (ed. Robards, A. W.), pp. 84–137. London: McGraw-Hill.Google Scholar
Morris, G. P. & Threadgold, L. T. (1968). Ultrastructure of the tegument of adult Schistosoma mansoni. Journal of Parasitology 54, 15–27.CrossRefGoogle ScholarPubMed
Oaks, J. A. & Lumsden, R. D. (1971). Cytological studies on the absorptive surface of cestodes. V. Incorporation of carbohydrate-containing macromolecules into tegument membranes. Journal of Parasitology 57, 1256–68.CrossRefGoogle ScholarPubMed
Perez, H. & Terry, R. J. (1973). The killing of adult Schistosoma mansoni in vitro in the presence of antisera to host antigenic determinants and peritoneal cells. International Journal for Parasitology 3, 499–503.CrossRefGoogle ScholarPubMed
Pitts, R. F. (1968). Physiology of the Kidney and Body Fluids. Chicago: Year Book Medical Publishers.Google Scholar
Ridderstrap, A. S. & Bonting, S. L. (1969). Na+−K+-activated ATPase and exocrine pancreatic secretion in vitro. American Journal of Physiology 217, 1721–7.CrossRefGoogle Scholar
Shannon, W. A. & Bogitsh, B. J. (1971). Megalodiscus temperatus: comparative radioautography of glucose-3H and galactose-3H incorporation. Experimental Parasitology 29, 309–19.CrossRefGoogle ScholarPubMed
Smithers, S. R., Terry, R. J. & Hockley, D. J. (1969). Host antigens in schistosomiasis. Proceedings of the Royal Society B 171, 483–94.Google ScholarPubMed
Wessels, N. K., Spooner, B. S., Ash, J. F., Bradley, M. O., Luduena, M. A., Taylor, E. L., Wrenn, J. T. & Yamada, K. M. (1971). Microfilaments in cellular and developmental processes. Science 171, 135–43.CrossRefGoogle Scholar
Wilson, R. A. & Barnes, P. E. (1974). The tegument of Schistosoma mansoni: observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function. Parasitology 68, 239–58.CrossRefGoogle ScholarPubMed