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An improved method to distinguish Entamoeba histolytica and Entamoeba dispar

Published online by Cambridge University Press:  01 October 1999

M. A. GOMES ,
J. B. PESQUERO ,
C. FURST ,
P. R. VALLE ,
J. L. PESQUERO  and
E. F. SILVA
M. A. GOMES
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP: 31270-901 Belo Horizonte, MG, Brazil
J. B. PESQUERO
Affiliation:
Departamento de Biofísica, Universidade Federal de São Paulo, R. Botucatu 862, CEP: 04023-062 São Paulo, SP, Brazil
C. FURST
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP: 31270-901 Belo Horizonte, MG, Brazil
P. R. VALLE
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP: 31270-901 Belo Horizonte, MG, Brazil
J. L. PESQUERO
Affiliation:
Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP: 31270-901 Belo Horizonte, MG, Brazil
E. F. SILVA
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP: 31270-901 Belo Horizonte, MG, Brazil
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Abstract

A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. The amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the DNA fragments. The method described here permits E. histolytica and E. dispar to be distinguished with greater sensitivity and rapidity.

Keywords

Entamoeba histolytica–Entamoeba dispar differentiationDNA electrophoresisDNA conformationdiagnosisPCR
Type
Research Article
Information
Parasitology , Volume 119 , Issue 4 , October 1999 , pp. 359 - 362
Copyright
1999 Cambridge University Press

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