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An improved method to distinguish Entamoeba histolytica and Entamoeba dispar

  • M. A. GOMES (a1), J. B. PESQUERO (a2), C. FURST (a1), P. R. VALLE (a1), J. L. PESQUERO (a3) and E. F. SILVA (a1)...

Abstract

A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. The amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the DNA fragments. The method described here permits E. histolytica and E. dispar to be distinguished with greater sensitivity and rapidity.

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Corresponding author

Corresponding author: Departamento de Parasitologia, ICB-UFMG, Av. Antonio Carlos 6627, 31270-901 Belo Horizonte, Minas Gerais, Brasil. Tel: +55 31 4992845. Fax: +55 31 4992970. E-mail: magomes@mono.icb.ufmg.br

Keywords

An improved method to distinguish Entamoeba histolytica and Entamoeba dispar

  • M. A. GOMES (a1), J. B. PESQUERO (a2), C. FURST (a1), P. R. VALLE (a1), J. L. PESQUERO (a3) and E. F. SILVA (a1)...

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