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Activity of two extracts of Cynanchum paniculatum against Ichthyophthirius multifiliis theronts and tomonts

  • WEN JI-HONG (a1), WANG YAN-LI (a1), LIU YU-HUA (a2), ZHANG JI-YUAN (a3) and LI ZE-HONG (a4)...

Summary

The present study aims to evaluate the antiparasitic activity of active components from Cynanchum paniculatum against Ichthyophthirius multifiliis. The antiparasitic activities of two bioassay-guided fractionationated compounds from C. paniculatum identified as Cynatratoside-A and Cynanversicoside C, by comparing spectral data (NMR and ESI-MS) with literature values, were evaluated by in vitro assay. These showed that both could kill theronts of I. multifiliis at a concentration of 10·0 mg L−1, with the median effective concentration (EC50) values of 4·6 mg L−1 and 5·2 mg L−1 for Cynatratoside-A and Cynanversicoside C, respectively. Encysted tomonts were killed at concentrations of 8·0 mg L−1 with both compounds. In vivo experiments demonstrated that fish treated with both compounds at 15·0 mg L−1 carried significantly fewer parasites than controls (P < 0·05). There were no mortalities among treated fish group compared with 75% mortality of untreated fish. Cynatratoside-A and Cynanversicoside C are therefore potential candidate drugs for use against I. multifiliis.

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Corresponding author

*Corresponding author. Agriculture University, 2888 Xincheng Road, Changchun, Jilin, China. E-mail: yaojiamm131@163.com

References

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