Hostname: page-component-848d4c4894-8kt4b Total loading time: 0 Render date: 2024-07-07T06:30:15.787Z Has data issue: false hasContentIssue false
  • English
  • Français

The action of amoebicidal drugs on Entamoeba invadens Rodhain, 1934 in vitro

Published online by Cambridge University Press:  06 April 2009

Elspeth W. McConnachie
Elspeth W. McConnachie
Affiliation:
Molteno Institute, University of Cambridge
Get access Rights & Permissions [Opens in a new window]

Extract

1. A method, based on that devised for E. histolytica by Dobell (1947), was worked out for testing the action of drugs upon E. invadens.

2. The sensitivity of di- and monobacterial strains of E. invadens to emetine was determined, and of a dibacterial strain to the quinaldines A 352, A 356 and A 404. E. invadens was killed by emetine at concentrations of 1/50,000–1/100,000, A 352 or A 356 at 1/100,000, and A 404 at 1/10,000. The strains of E. invadens used in the present tests are thus less sensitive to emetine than is E. histolytica, but the sensitivity of the two species to the quinaldines is similar.

3. A strain of E. invadens which was maintained for 9 months in the presence of increasing concentrations of emetine, did not become resistant to the drug.

I am indebted to Dr A. Bishop for much advice and criticism; to Dr J. D. Fulton for a culture of Bacterium coli A'; and to Dr J. Walker for supplies of the compounds A 352, A 356 and A404. The work has been financed by the Medical Research Council.

Type
Research Article
Information
Parasitology , Volume 44 , Issue 1-2 , May 1954 , pp. 132 - 143
Copyright
Copyright © Cambridge University Press 1954

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, H. H. & Chuan, T. T. K. (1944). Comparative amebacidal activity of phenyl arsine oxide (Mapharsen), related arsenicals and other agents. Amer. J. Trop. Med. 24, 367.CrossRefGoogle Scholar
Bishop, A. (1929). Experiments upon the action of emetine in cultures of Entamoeba coli. Parasitology, 21, 481.CrossRefGoogle Scholar
Bonnin, H. & Aretas, R. (1938). Entamoeba dysenteriae et émétine in vitro. Essais d'émétine. Résistance provoquée. Bull. Soc. Pat. exot. 31, 829.Google Scholar
Bonnin, H. & Aretas, R. (1939). Résistance acquise des amibes (Entamoeba dysenteriae) à l'émétine in vitro. C.R. Soc. Biol., Paris, 130, 495.Google Scholar
Conan, N. J. (1948). Chloroquine in amebiasis. Amer. J. Trop. Med. 28, 107.CrossRefGoogle ScholarPubMed
Dobell, C. (1947). An improved method for testing the action of emetine and other chemicals on Entamoeba histolytica in cultures. Ann. Soc. belge Méd. trop. (Liber jubilaris J. Rodhain), 27, suppl., 201.Google Scholar
Dobell, C. (with Neal, R. A. & Hoare, C. A.), (1952). Researches on the intestinal Protozoa of monkeys and man. XII. Bacterial factors influencing the life history of Entamoeba histolytica in cultures. Parasitology, 42, 16.CrossRefGoogle ScholarPubMed
Dobell, C. & Laidlaw, P. P. (1926). The action of ipecacuanha alkaloids on Entamoeba histolytica and some other entozoic amoebae in culture. Parasitology, 18, 206.CrossRefGoogle Scholar
Fulton, J. D., Joyner, L. P., King, H., Osbond, J. M. & Wright, J. (1950). Amoebicidal action and chemical constitution. Proc. Roy. Soc. B, 137, 339.Google ScholarPubMed
Geiman, Q. M. & Ratcliffe, H. L. (1936). Morphology and life-cycle of an amoeba producing amoebiasis in reptiles. Parasitology, 28, 208.CrossRefGoogle Scholar
Halawani, A. (1930). Experimental study on the resistance of Entamoeba histolytica to emetine hydrochloride in vitro. Ann. Trop. Med. Parasit. 24, 273.CrossRefGoogle Scholar
Hansen, E. L. (1949). Amebacidal action of agents inhibitory to associated bacteria in vitro. Fed. Proc. 8, 299.Google Scholar
Ishii, N. (1948). Researches on the chemotherapy of protozoal infections. Jap. Med. J. 1, 30.CrossRefGoogle ScholarPubMed
Jones, W. R. (1952). Experimental attempt to induce drug-resistance in Entamoeba histolytica. Exp. Parasit. 1, 118.CrossRefGoogle Scholar
Laidlaw, P. P., Dobell, C. & Bishop, A. (1928). Further experiments on the action of emetine in cultures of Entamoeba histolytica. Parasitology, 20, 207.CrossRefGoogle Scholar
Lamy, L. & Chevrier, A. M. (1948). Action comparée de l'(P-amino-benzènsulfamido)-pyridine sur deux amibes parasites. Bull. Soc. Pat. exot. 41, 149.Google Scholar
Nakamura, M. & Anderson, H. H. (1951). Evidence of direct amebacidal action of agents tested in vitro against Endamoeba histolytica. J. Parasit. 37, 421.CrossRefGoogle Scholar
Phillips, B. P. (1950). Cultivation of Endamoeba histolytica with Trypanosoma cruzi. Science, 111, 8.CrossRefGoogle ScholarPubMed
Phillips, B. P. & Rees, C. W. (1950). The growth of Endamoeba histolytica with live and heat-treated Trypanosoma cruzi. Amer. J. Trop. Med. 30, 185.CrossRefGoogle ScholarPubMed
Ratcliffe, H. L. & Geiman, Q. M. (1933). Eleven cases of amoebiasis in reptiles. J. Parasit. 20, 139.Google Scholar
Ratcliffe, H. L. & Geiman, Q. M. (1934). Amebiasis in reptiles. Science, 79, 324.CrossRefGoogle ScholarPubMed
Ratcliffe, H. L. & Geiman, Q. M. (1938). Spontaneous and experimental amebic infection in reptiles. Arch. Path. 25, 160.Google Scholar
Rodhain, J. (1934). Entamoeba invadens n.sp., parasite de serpents. C.R. Soc. Biol., Paris, 117, 1195.Google Scholar
Rodhain, J. & Van Hoof, M. T. (1935). Sur le rôle pathogène d'Entamoeba invadens. C.R. Soc. Biol., Paris, 118, 1646.Google Scholar
St John, J. H. (1933). The effect of emetin on Endamoeba histolytica in culture. Amer. J. Hyg. 18, 414.Google Scholar
Stewart, G. T. (1949). Nature of the action of emetine upon Entamoeba histolytica. Nature, Lond., 163, 842.CrossRefGoogle ScholarPubMed