Hostname: page-component-77c89778f8-fv566 Total loading time: 0 Render date: 2024-07-17T16:28:20.098Z Has data issue: false hasContentIssue false
  • English
  • Français

The water dynamics of stages of Ditylenchus dipsaci and D. myceliophagus during desiccation and rehydration

Published online by Cambridge University Press:  06 April 2009

R. N. Perry
R. N. Perry
Affiliation:
Department of Zoology, The University, Newcastle upon Tyne.
Get access Rights & Permissions [Opens in a new window]

Extract

Interference microscopy has been used to study the rate at which water enters and is lost from larval and adult stages of Ditylenchus dipsaci and D. myceliophagus. It is shown that the cuticle of the dry nematode, irrespective of stage, is completely permeable to water. In contrast, marked intra– and interspecific differences were found in the rate at which stages dry at 0 and 50% relative humidity. All stages of D. myceliophagus dry very rapidly: in this species, death, as a consequence of severe desiccation, appears to be linked to a lack of any marked ability to control water loss. For D. dipsaci, those stages known to survive desiccation will appear to be the slower dryers. In general, the “order of performance” in slowing the rate of drying is the same as the order previously found for survival of desiccation. Both the 4th– and, to a lesser extent, the 3rd–stage larvae of D. dipsaci show evidence of an intrinsic ability to control water loss.

An analysis of the drying curve for 4th–stage larvae of D. dipsaci supports the indications of interference photographs that the rate of drying is slowed by a decrease in permeability of the drying cuticle.

Vacuum desiccation studies further demonstrate the remarkable ability of the 4th–stage larva of D. dipsaci to control its rate of drying. The survival of 4th– and 3rd–stage larvae of D. dipsaci is diminished by very rapid drying, and the survival of other stages of this species and larval and adult stages of D. myceliophagus is precluded. The decrease in survival of the 4th–stage larvae of D. dipsaci is shown to be associated with a more rapid rate of water loss.

Type
Research Article
Information
Parasitology , Volume 75 , Issue 1 , August 1977 , pp. 45 - 70
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Beament, J. W. L. (1964). The active transport of water; evidence, models and mechanisms. Symposium of the Society for Experimental Biology 19, 273–97.Google Scholar
Cairns, E. J. (1953). Pathology and biology of Ditylenchus sp. in relation to a disease of Agaricus campestris L. Thesis, University of Maryland, USA.Google Scholar
Ellenby, C. (1968 a). Desiccation survival in the plant parasitic nematodes, Heterodera rostochiensis Wollenweber and Ditylenchus dipsaci (Kühn). Proceedings of the Royal Society of London, B 169, 203–13.Google Scholar
Ellenby, C. (1968 b). Desiccation survival of the infective larva of Haemonchus contortus. Journal of Experimental Biology 40, 469–75.CrossRefGoogle Scholar
Ellenby, C. (1968 c). Determination of the water content of nematode worms by interference microscopy. Experientia 24, 84–5.CrossRefGoogle ScholarPubMed
Ellenby, C. (1968 d). The survival of desiccated larvae of Heterodera rostochiensis and H. schachtii. Nematologica 14, 544–8.CrossRefGoogle Scholar
Ellenby, C. (1969). Dormancy and survival in nematodes. Symposium of the Society for Experimental Biology 23, 83–97.Google Scholar
Ellenby, C. & Perry, R. N. (1976). The influence of the hatching factor on the water uptake of the second stage larva of the potato cyst nematode Heterodera rostochiensis. Journal of Experimental Biology 64, 141–7.CrossRefGoogle Scholar
Goodey, J. B. (1958). Ditylenchus myceliophagus n.sp. (Nematoda: Tylenchidae). Nematologica 3, 91–6.CrossRefGoogle Scholar
Lees, E. (1953). An investigation into the method of dispersal of Panagrellus silusiae, with particular reference to its desiccation resistance. Journal of Helminthology 27, 95–103.CrossRefGoogle Scholar
Palo, A. V. (1962). Translocation and development of stem eelworm, Ditylenchus dipsaci (Kühn) in lucerne, Medicago sativa L. Nematologica 7, 122–32.CrossRefGoogle Scholar
Perry, R. N. (1975). Desiccation survival of Ditylenchus dipsaci (Kühn, 1857) Filipjev, 1936 and D. myceliophagus J. B. Goodey 1958. Ph.D. thesis, University of Newcastle upon Tyne.Google Scholar
Perry, R. N. (1976). Separation of stages of Ditylenchus dipsaci and D. myceliophagus by body length. Nematologica 22, 446–50.CrossRefGoogle Scholar
Perry, R. N. (1977 a). Desiccation survival of larval and adult stages of the plant parasitic nematodes, Ditylenchus dipsaci and D. myceliophagus. Parasitology 74, 139–48.CrossRefGoogle Scholar
Perry, R. N. (1977 b). The effect of previous desiccation on the ability of the 4th stage larva of D. dipsaci to survive drying and control its rate of water loss. Parasitology (in the Press).CrossRefGoogle Scholar
Steel, R. G. D. & Torrie, J. H. (1960). Principles and Procedures of Statistics. new York: McGraw-Hill.Google Scholar
Webster, J. M. (1964). The effect of storage conditions on the infectivity of narcissus stem eelworm. Plant Pathology 13, 151–4.CrossRefGoogle Scholar