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Ultrastructural studies on the sporogony of Hepatozoon spp. in Culex quinquefasciatus Say, 1823 fed on infected Caiman crocodilus and Boa constrictor from northern Brazil

Published online by Cambridge University Press:  09 October 2003

I. PAPERNA  and
R. LAINSON
I. PAPERNA
Affiliation:
Department of Animal Sciences, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76-100, Israel
R. LAINSON
Affiliation:
Department of Parasitology, The Instituto Evandro Chagas, Avenida Almirante Barroso 492, Belem 66090-000, Para, Brazil
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Abstract

Laboratory reared Culex quinquefasciatus Say, 1823 originating from the vicinity of Belem, in northern Brazil, were allowed to engorge on caimans (Caiman c. crocodilus) infected with Hepatozoon caimani (Carini, 1909) and boas (Boa constrictor) infected with H. cf. terzii (Sambon and Seligmann, 1907) both from Para State. Engorged mosquitoes killed on successive days post-feeding (p.f.) were studied by transmission electron microscopy. Images of oocysts from 13 days p.f., caiman-fed mosquitoes were also examined by scanning electron microscopy. The Hepatozoon spp. from the respective hosts differed in their ability to develop in C. quinquefasciatus. Most female mosquitoes fed on caimans, became fully engorged, and survived beyond 22 days p.f., whereas those engorged on boa became debilitated and did not survive beyond 9 days p.f. In boa-fed mosquitoes oocysts were found on the 6th day p.f. The few mosquitoes surviving to the 9th day p.f. contained either undivided oocysts or those that had already commenced sporogenic division. By 8–10 days p.f. caiman-fed mosquitoes contained uninucleate oocysts. Sporogonic divisions were observed from day 12 p.f. onwards. Although sporogenic development conformed in general with the previously reported accounts, the study allowed a more detailed examination of the plasmalemmal endocytotic system, and the consolidation of the crystalline body in specialized ‘factories’ of cystalline material. Sporozoite differentiation occasionally started on the 18th day p.f., but otherwise was observed only on day 22 p.f.

Keywords

HepatozoonCaiman crocodilusBoa constrictorCulex quinquefasciatusexperimental infectionultrastructureBrazil
Type
Research Article
Information
Parasitology , Volume 127 , Issue 2 , August 2003 , pp. 147 - 154
Copyright
2003 Cambridge University Press

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